ykuN

ykuN
168

flavodoxin, binds FMN, replaces ferredoxin under conditions of iron limitation, probably involved in electron transfer to nitric oxide synthase

locus
BSU_14150
Molecular weight
17.65 kDa
pI
3.83
Protein length
Gene length
function
electron transfer
product
flavodoxin
essential
no
synonyms
ykuN

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0716 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,487,038  1,487,514
The protein
Protein family
flavodoxin family (with [protein|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|ykuP], according to UniProt)
[wiki|Domains]
[wiki|Flavodoxin-like domain] (aa 4-144) (according to UniProt)
[wiki|Cofactors]
FMN [Pubmed|25194416]
Structure
[PDB|5LJL] (flavodoxin from Streptococcus pneumoniae, 42% identity) [pubmed|27649488]
[AF|O34737]
Paralogous protein(s)
[protein|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|ykuP]
Expression and Regulation
Operons
genes
[gene|0932DDC285635C02067A3302CEDA302FE4269179|ykuN]-[gene|CEC1B5DF1E548597FE333895ECA476AB9DFFFDD7|ykuO]-[gene|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|ykuP]
description
[Pubmed|12354229]
regulation
expressed under anaerobic conditions ([protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]) [Pubmed|24214949]
induced by iron starvation (second wave) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]) [pubmed|29133393,12354229]
regulatory mechanism
[protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]: activation, [Pubmed|24214949], in [regulon|protein:1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD regulon]
[protein|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|kre]: repression, in [regulon|protein:65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|kre regulon]
[protein|EC6697D5D945B7E5083AFED9218748763C443278|nsrR]: repression, [Pubmed|24214949], in [regulon|protein:EC6697D5D945B7E5083AFED9218748763C443278|nsrR regulon]
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12354229], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI]: sigma factor, [pubmed|29914988], in [regulon|protein:3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI regulon]
additional information
the ''[protein|0932DDC285635C02067A3302CEDA302FE4269179|ykuN]-[protein|CEC1B5DF1E548597FE333895ECA476AB9DFFFDD7|ykuO]-[protein|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|ykuP]'' operon is strongly unregulated in a ''[wiki|kre]'' mutant [Pubmed|26110430]
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[gene|0932DDC285635C02067A3302CEDA302FE4269179|ykuN]→[gene|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|ykuP]

2024-11-08 13:41:11

Jstuelk

87

57db5b9bbc0032750db2eb9bfdbed1110c747af0

E599E3DBF7B4049028BE0EE09C6A470E8B1A923E

Biological materials
Mutant
GP4733 Δ[gene|0932DDC285635C02067A3302CEDA302FE4269179|ykuN]::aphA3, available in [wiki|Jörg Stülke]'s lab
MGNA-B337 (ykuN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1336 NBRP B. subtilis, Japan]
BKE14150 ([gene|0932DDC285635C02067A3302CEDA302FE4269179|ykuN]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14150 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTATCACCCCATTAGT,  downstream forward: _UP4_GATTATATGAACAAGGAAAA
BKK14150 ([gene|0932DDC285635C02067A3302CEDA302FE4269179|ykuN]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14150 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTATCACCCCATTAGT,  downstream forward: _UP4_GATTATATGAACAAGGAAAA
References
36815589,15449930,17127770,12354229,20186410,24214949,21665975,25194416,25933252,26110430,28439033,27649488,29133393,29914988,31113899,39481744

0932DDC285635C02067A3302CEDA302FE4269179

Page visits: 6026

Time of last update: 2024-12-06 17:46:50

Author of last update: Jstuelk