bceS
168
two-component sensor kinase, regulation of [gene|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA]-[gene|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB] in response to bacitracin, plectasin, mersacidin and actagardine
locus
BSU_30390
Molecular weight
38.61 kDa
pI
5.94
function
resistance against toxic peptides
product
two-component sensor kinase
essential
no
synonyms
bceS, ytsB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0642 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,112,190 3,113,194
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of [protein|C239C155F5452C86BF6C78190BABBBB07A63DF87|bceR]
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
[wiki|Domains]
two transmembrane segments, C-terminal histidine phosphotransferase domain
[wiki|Histidine kinase domain] (aa 121-326) (according to UniProt)
Structure
[PDB|8G3A] ([protein|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA]-[protein|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB]-[protein|08F24CA21DC54031F6158AC0C772F3B520E5A849|bceS]) [pubmed|37393310]
[AF|O35044]
Modification
autophosphorylation on a His residue
Effectors of protein activity
binding of [protein|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB] in the absence of bacitracin inhibits [protein|08F24CA21DC54031F6158AC0C772F3B520E5A849|bceS] autophosphorylation [Pubmed|25118291]
[wiki|Localization]
cell membrane (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|C239C155F5452C86BF6C78190BABBBB07A63DF87|bceR]-[gene|08F24CA21DC54031F6158AC0C772F3B520E5A849|bceS]
description
[Pubmed|12399481]
Biological materials
Mutant
GP3218 Δ([gene|C239C155F5452C86BF6C78190BABBBB07A63DF87|bceR]-[gene|08F24CA21DC54031F6158AC0C772F3B520E5A849|bceS]-[gene|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA]-[gene|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB])::''cat'', available in [wiki|Jörg Stülke]'s lab
MGNA-A166 (ytsB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/166 NBRP B. subtilis, Japan]
BKE30390 ([gene|08F24CA21DC54031F6158AC0C772F3B520E5A849|bceS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCTCCGCCTTTCGATAAGGA, downstream forward: _UP4_TGACGAAAATGTCACATGCT
BKK30390 ([gene|08F24CA21DC54031F6158AC0C772F3B520E5A849|bceS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCTCCGCCTTTCGATAAGGA, downstream forward: _UP4_TGACGAAAATGTCACATGCT
References
Reviews
Original Publications
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Time of last update: 2024-12-22 00:24:40
Author of last update: Jstuelk