ispH

ispH
168

(E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 7th step in the MEP pathway of isoprenoid biosynthesis

locus
BSU_25160
Molecular weight
34.77 kDa
pI
5.61
Protein length
Gene length
function
MEP pathway of isoprenoid biosynthesis
product
(E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase
essential
yes
ec
1.17.1.2
synonyms
ispH, yqfP

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0761 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,596,535  2,597,479
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
H2O + isopentenyl diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] --> (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H+ + 2 reduced [2Fe-2S]-[ferredoxin] (according to UniProt)
dimethylallyl diphosphate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] --> (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H+ + 2 reduced [2Fe-2S]-[ferredoxin] (according to UniProt)
Protein family
ispH family (single member, according to UniProt)
[wiki|Cofactors]
Fe-S cluster [pubmed|29292548]
Structure
[PDB|3F7T] (IspH from ''E. coli'', 38% identity) [Pubmed|19569147]
[AF|P54473]
Expression and Regulation
Operons
genes
[gene|9B1EA9872500280D6360EECD3D7344A1974910CA|yqfQ]-[gene|08F1D9AD2D51D8513AFFA57E8ED69DB8EF97AD88|ispH]
description
[Pubmed|22383849]
Open in new tab

[gene|9B1EA9872500280D6360EECD3D7344A1974910CA|yqfQ]→[gene|08F1D9AD2D51D8513AFFA57E8ED69DB8EF97AD88|ispH]

2024-11-19 19:13:07

Jstuelk

85

69a836a329d5d1e40c1af32959f1da5b11f0b538

1AAA1FC08E493CDD5C6CB96902A62A4C0BFF8632

genes
[gene|08F1D9AD2D51D8513AFFA57E8ED69DB8EF97AD88|ispH]
description
[Pubmed|22383849]
Open in new tab

[gene|08F1D9AD2D51D8513AFFA57E8ED69DB8EF97AD88|ispH]

2024-11-02 13:52:17

Jstuelk

73

5f335d8e0b43c6d367844a714996f3605c71ac7f

3742286ACEBFF3090EDE7DCD528354A68A0DB2A2

References
17458547,19569147,23840410,27791134

08F1D9AD2D51D8513AFFA57E8ED69DB8EF97AD88

Page visits: 5022

Time of last update: 2024-12-07 05:00:43

Author of last update: Melvin.boenninger