yisK
168
oxaloacetate enol-keto tautomerase, modulator of [protein|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl] activity, minor oxaloacetate decarboxylase activity
locus
BSU_10750
Molecular weight
32.99 kDa
pI
5.45
function
detoxification of enol-oxaloacetate, control of [wiki|cell division],
product
oxaloacetate enol-keto tautomerase, modulator of [protein|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl] activity
essential
no
ec
5.3.2.2
synonyms
yisK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0179 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,152,244 1,153,149
Phenotypes of a mutant
overexpression results in loss of cell width and cell lysis (suppressed by mutations in [protein|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl] that affect the ATP-binding site) [Pubmed|27215790]
The protein
Catalyzed reaction/ biological activity
enol-oxaloacetate --> oxaloacetate [pubmed|38287013]
oxaloacetate --> pyruvate + CO2 [pubmed|38047707]
Protein family
FAH family (single member, according to UniProt)
[wiki|Cofactors]
Mn2+ [pubmed|38047707]
Structure
[PDB|3R60] (from ''Mycobacterium abscessus'', 42% identity)
[PDB|8SKY] [pubmed|38047707]
[AF|O06724]
Kinetic information
for oxaloacetate tautomerase activity
Km = 44 +/- 8 µM [pubmed|38287013]
vmax = 402 +/- 13 µmol min-1 mg-1 [pubmed|38287013]
for oxaloacetate decarboxylase activity
Km = 134 µM [pubmed|38047707]
Kcat = 31 min−1 [pubmed|38047707]
[wiki|Localization]
punctate and/or punctate/helical pattern, depends on [protein|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl] [pubmed|38047707]
Expression and Regulation
Operons
genes
[gene|07E876715DCF551B8BDB6AA5D33C700696B4E730|yisK]-[gene|BDA8B49AC4AE81383D84337C7FEE9D88EA92AED3|yisL]
description
[pubmed|22383849]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, [Pubmed|27215790], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
sigma factors
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|27215790], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
Biological materials
Mutant
MGNA-B291 (yisK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1290 NBRP B. subtilis, Japan]
BKE10750 ([gene|07E876715DCF551B8BDB6AA5D33C700696B4E730|yisK]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTATTCCTCCATCATC, downstream forward: _UP4_TGAATAAAACTGGAGGGCGG
BKK10750 ([gene|07E876715DCF551B8BDB6AA5D33C700696B4E730|yisK]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTATTCCTCCATCATC, downstream forward: _UP4_TGAATAAAACTGGAGGGCGG
References
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Time of last update: 2025-03-15 00:23:20
Author of last update: Jstuelk