fmt
168
methionyl-tRNA formyltransferase
locus
BSU_15730
Molecular weight
34.48 kDa
pI
5.62
function
formylation of Met-tRNA(fMet)
product
methionyl-tRNA formyltransferase
essential
no
ec
2.1.2.9
synonyms
fmt, yloL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0223 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,646,999 1,647,952
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
inactivation of ''[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]'' suppresses the synthetic lethality of the ''[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA] [gene|53B760316B19E142FC3ECAD2CFAA540377B08BBC|defB]'' double mutant [Pubmed|27983482]
essential according to Kobayashi et al. [Pubmed|12682299], but a deletion mutant was constructed by Cai et al. [Pubmed|27983482]
the mutant is defective for [wiki|biofilm formation], motility, and [wiki|sporulation] [Pubmed|27983482]
the mutant exhibits increased sensitivity to metal ion excess and oxidative stress [Pubmed|27983482]
The protein
Catalyzed reaction/ biological activity
(6S)-10-formyltetrahydrofolate + L-methionyl-tRNAfMet --> (6S)-5,6,7,8-tetrahydrofolate + H+ + N-formyl-L-methionyl-tRNAfMet (according to UniProt)
Protein family
fmt family (single member, according to UniProt)
Structure
[PDB|3RFO] (from ''Bacillus anthracis'', 69% identity, 89% similarity)
[AF|P94463]
Expression and Regulation
Operons
genes
[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]
description
[Pubmed|16964327]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16964327], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]-[gene|2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C|coaBC]-[gene|AF5ADDA2A98EC18BB72A81AA0336E639C0D1F430|priA]-[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]
description
[pubmed|22383849]
additional information
[protein|search|translation] is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]
Biological materials
Mutant
BKE15730 ([gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAATACGATTCTCGTCA, downstream forward: _UP4_GCCGGTGATGTGTTAGGAGT
BKK15730 ([gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAATACGATTCTCGTCA, downstream forward: _UP4_GCCGGTGATGTGTTAGGAGT
References
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Time of last update: 2024-12-21 09:12:31
Author of last update: Christoph.elfmann