ydeL
168
similar to transcriptional regulator ([wiki|MocR/ GabR family])
locus
BSU_05240
Molecular weight
53.36 kDa
pI
9.22
function
unknown
product
unknown
essential
no
synonyms
ydeL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1167 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
571,389 572,780
The protein
Protein family
[wiki|MocR/ GabR family ][Pubmed|22020104]
[wiki|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
[wiki|Domains]
N-terminal DNA-binding helix-turn-helix motif; C-terminal domain is homologous to PLP-binding large domain of aminotransferases.
[wiki|HTH gntR-type domain] (aa 13-81) (according to UniProt)
[wiki|Cofactors]
PLP
Structure
[PDB|4MGR] ([protein|C7C36FAC0CE72226960FC7E8E016B8EC77AF1036|gabR], 38% identity) [pubmed|24127574]
[AF|P96669]
[wiki|Localization]
nucleoid (Homogeneous) [Pubmed|16479537]
Biological materials
Mutant
MGNA-C083 (ydeL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2081 NBRP B. subtilis, Japan]
BKE05240 ([gene|05A23F4611674B0092262DEC309F7AD6B9B5BE30|ydeL]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGCGCGTTGTTCTCCT, downstream forward: _UP4_TGAGGGAGCTTTCAGTTTAG
BKK05240 ([gene|05A23F4611674B0092262DEC309F7AD6B9B5BE30|ydeL]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGCGCGTTGTTCTCCT, downstream forward: _UP4_TGAGGGAGCTTTCAGTTTAG
Expression vectors
pGP3937 (N-terminal Strep-tag, purification from E. coli, in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-11-21 09:01:42
Author of last update: Dennis.wicke