lipC
168
spore coat protein, phospholipase implicated in spore germination
locus
BSU_04110
Molecular weight
23.46 kDa
pI
5.84
function
spore germination
product
spore coat phospholipase B
essential
no
synonyms
lipC, ycsK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2755 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
462,431 463,072
Phenotypes of a mutant
defective in L-alanine-stimulated germination [Pubmed|20057119]
The protein
Catalyzed reaction/ biological activity
cleaves the fatty acids at the sn-1 and sn-2 positions of phospholipids [Pubmed|20057119]
Protein family
[wiki|'GDSL' lipolytic enzyme family] (according to UniProt)
Structure
[PDB|4PPY] (from Bacteroides fragilis, corresponds to aa 41 ... 210, 25.4% identity)
[AF|P42969]
[wiki|Localization]
spore coat (basement), localization depends on [protein|A25C1530DA7BB007A288E525404E9F775E219FE8|spoIVA] [Pubmed|22171814,17220230,20057119]
Expression and Regulation
Operons
genes
[gene|05910B4500420D4DB75DAD3A7A2358C360FF2154|lipC]
description
(according to [http://dbtbs.hgc.jp/COG/prom/ycsFGI-kipIAR-ycsK.html DBTBS]) null
regulation
expressed four hours after onset of [wiki|sporulation ]([protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]) [Pubmed|17220230]
regulatory mechanism
[protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]: activation, [Pubmed|17220230], in [regulon|protein:19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE regulon]
sigma factors
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15699190,17220230], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
genes
[gene|77F758CE9A59AC41F283CB1F5099097386133799|pxpA]-[gene|9AD419E3CB86C54A0F11E96C82E80A357C6B23D2|ycsG]-[gene|312E2A4ADFBCB9DD9AD9EEEC9FE53F3932AF5DBA|ycsI]-[gene|5A5E17D39296FECC1C03B1A7791AB1065BB4E4DF|pxpB]-[gene|3BA8F1F71B1D65EB30867ABD27867F925DAD2D7B|pxpC]-[gene|7DA9A79876C546B78B716A64706A3A3716018C2E|kipR]-[gene|05910B4500420D4DB75DAD3A7A2358C360FF2154|lipC]
description
[Pubmed|9334321]
regulation
induced in the presence of 5-oxoproline [pubmed|28830929]
regulatory mechanism
[protein|7DA9A79876C546B78B716A64706A3A3716018C2E|kipR]: repression, [Pubmed|9334321], in [regulon|protein:7DA9A79876C546B78B716A64706A3A3716018C2E|kipR regulon]
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [Pubmed|9334321], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
Biological materials
Mutant
MGNA-C024 (ycsK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2022 NBRP B. subtilis, Japan]
BKE04110 ([gene|05910B4500420D4DB75DAD3A7A2358C360FF2154|lipC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTTTCCTCCACTCT, downstream forward: _UP4_TAGAAAACGCGCCTCATGAC
BKK04110 ([gene|05910B4500420D4DB75DAD3A7A2358C360FF2154|lipC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTTTCCTCCACTCT, downstream forward: _UP4_TAGAAAACGCGCCTCATGAC
References
Reviews
Original Publications
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Time of last update: 2024-12-20 22:07:17
Author of last update: Jstuelk