rnr
168
RNase R, required for protection against paraquate stress
locus
BSU_33610
Molecular weight
88.56 kDa
pI
5.7
function
nonspecific degradation of rRNA
product
exoribonuclease RNase R (EC 3.1.-.-)
essential
no
synonyms
rnr, yvaJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0557 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,451,863 3,454,202
Phenotypes of a mutant
defective in [category|SW.4.1.4|Swarming] motility (prolonged period of immotility prior to [category|SW.4.1.4|Swarming]) [pubmed|35638827]
The protein
Catalyzed reaction/ biological activity
3'-5'-exoribonuclease
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates (according to UniProt)
Protein family
RNR ribonuclease family (single member, according to UniProt)
[wiki|Domains]
[wiki|S1 domain] (aa 628-708) (according to UniProt)
2 [wiki|CSD domain]s (aa 73-133, aa 138-202) (according to the Interpro database)
Structure
[PDB|5XGU] (from E. coli, 40% identity) [pubmed|29036353]
[AF|O32231]
Expression and Regulation
Operons
genes
[gene|D8851F453E5EF18D36F25834A8CB9C61EAF15E44|secG]-[gene|C0B6520E74CE9F7EE3DEC8C0D83E0770F241C4E8|yvaK]-[gene|0472A4346ADA1DC41C9D00E8F3222A0EBB76DFA8|rnr]-[gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB]-[gene|5C12BB19B975F7FEBCFA119DAC66338C20FCECFA|ssrA]
description
[Pubmed|17369301]
regulation
''[protein|search|yvaK]'': induced by stress ([protein|search|SigB]) [Pubmed|11544224]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|11544224], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
genes
[gene|C0B6520E74CE9F7EE3DEC8C0D83E0770F241C4E8|yvaK]-[gene|0472A4346ADA1DC41C9D00E8F3222A0EBB76DFA8|rnr]-[gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB]-[gene|5C12BB19B975F7FEBCFA119DAC66338C20FCECFA|ssrA]
description
[Pubmed|17369301]
regulation
''[protein|search|yvaK]'': induced by stress ([protein|search|SigB]) [Pubmed|11544224]
Biological materials
Mutant
MGNA-A495 (yvaJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/495 NBRP B. subtilis, Japan]
BKE33610 ([gene|0472A4346ADA1DC41C9D00E8F3222A0EBB76DFA8|rnr]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCCATTATCCGACCTCCTG, downstream forward: _UP4_TAGCAGCTCAACCAGCAAAT
BKK33610 ([gene|0472A4346ADA1DC41C9D00E8F3222A0EBB76DFA8|rnr]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCCATTATCCGACCTCCTG, downstream forward: _UP4_TAGCAGCTCAACCAGCAAAT
labs
[wiki|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers%20Laboratories%20and%20Programs/Bechhofer%20Laboratory?citype=Physician&ciid=Bechhofer%20David%20H%201255565 Homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-25 21:39:01
Author of last update: Jstuelk