bglS
168
endo-beta-1,3-1,4 glucanase
locus
BSU_39070
Molecular weight
27.12 kDa
pI
6.48
function
lichenan degradation
product
endo-beta-1,3-1,4 glucanase
essential
no
ec
3.2.1.73
synonyms
bglS, bgl, licS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2273 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,011,842 4,012,570
The protein
Catalyzed reaction/ biological activity
Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds (according to UniProt)
Protein family
glycosyl hydrolase 16 family (single member, according to UniProt)
[wiki|Domains]
GH16 domain (aa 29-242) (according to UniProt)
Structure
[PDB|3O5S]
[AF|P04957]
[wiki|Localization]
extracellular (signal peptide) [Pubmed|18957862]
Expression and Regulation
Operons
genes
[gene|044A0EBC8798B110E719B73664EB80F53112D8DE|bglS]
description
[Pubmed|8606172]
regulation
expressed in the stationary phase (temporal activation) [Pubmed|8245830]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|8245831], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT]: antitermination, via binding to an [wiki|RNA switch], in [regulon|protein:FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8606172], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT]-[gene|044A0EBC8798B110E719B73664EB80F53112D8DE|bglS]
description
[Pubmed|8606172]
regulation
expressed in the stationary phase (temporal activation) [Pubmed|8245830]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8606172], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|6BC5A936D8EDE7087C3F6293A2873EC5352FE5EF|yxiP]-[gene|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT]-[gene|044A0EBC8798B110E719B73664EB80F53112D8DE|bglS]
description
[Pubmed|22383849]
Biological materials
Mutant
GP427 ([gene|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|licT]-[gene|044A0EBC8798B110E719B73664EB80F53112D8DE|bglS]::erm), BGW7 (cat), both available in [wiki|Jörg Stülke]'s lab
BKE39070 ([gene|044A0EBC8798B110E719B73664EB80F53112D8DE|bglS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGGCATTCCCCTTTC, downstream forward: _UP4_TAATGCCAAATGTGAAAGAG
BKK39070 ([gene|044A0EBC8798B110E719B73664EB80F53112D8DE|bglS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGGCATTCCCCTTTC, downstream forward: _UP4_TAATGCCAAATGTGAAAGAG
References
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Time of last update: 2025-04-28 08:59:31
Author of last update: Jstuelk