ligA
168
DNA ligase (NAD-dependent)
locus
BSU_06620
Molecular weight
74.69 kDa
pI
4.71
function
replication
product
DNA ligase (NAD-dependent)
essential
yes
ec
6.5.1.2
synonyms
ligA, lig, yerG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0272 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
721,613 723,619
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
NAD(+) + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) --> (deoxyribonucleotide)(n+m) + AMP + beta-nicotinamide D-nucleotid (according to UniProt)
Protein family
NAD-dependent DNA ligase family (single member, according to UniProt)
[wiki|Domains]
BRCT domain (aa 590-668) (according to UniProt)
Structure
[PDB|1B04] (adenylation domain, Geobacillus stearothermophilus)
[AF|O31498]
Expression and Regulation
Operons
genes
[gene|0BA7EC658F5C2397A90B68AC234D04966966561C|pcrB]-[gene|C73E52D214E24F21696973B19B0A44CE785D6FBA|pcrA]-[gene|03EFF93C1B0CE075AE068ECC4EDE9BE5D03C857E|ligA]-[gene|ADAE6B5C301959D90592A6F26DFE1690BF6F5552|yerH]
description
[Pubmed|9701819]
regulation
induced by DNA damage ([protein|search|LexA]) [Pubmed|16267290]
regulatory mechanism
[protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]
Biological materials
Mutant
MGNA-B444 (yerG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1443 NBRP B. subtilis, Japan]
References
Reviews
Original Publications
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Time of last update: 2024-12-18 10:24:38
Author of last update: Jstuelk