cspR
168
tRNA (Um34/Cm34) methyltransferase
locus
BSU_08930
Molecular weight
18.44 kDa
pI
6.05
function
translation
product
tRNA (Um34/Cm34) methyltransferase
essential
no
ec
2.1.1.-
synonyms
cspR, ygaR, trmL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0219 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
970,135 970,617
Phenotypes of a mutant
essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]
The protein
Catalyzed reaction/ biological activity
catalyzes the methyl transfer from S-adenosyl-L-methionine to tRNA(Leu)(CAA) and tRNA(Leu)(UAA) isoacceptors [Pubmed|23804755]
cytidine34 in tRNA + S-adenosyl-L-methionine --> 2'-O-methylcytidine34 in tRNA + H+ + S-adenosyl-L-homocysteine (according to UniProt)
5-carboxymethylaminomethyluridine34 in tRNALeu + S-adenosyl-L-methionine --> 5-carboxymethylaminomethyl-2'-O-methyluridine34 in tRNALeu + H+ + S-adenosyl-L-homocysteine (according to UniProt)
Protein family
[wiki|Methyltransferase superfamily] (according to UniProt)
class IV-like SAM-binding methyltransferase superfamily (with [protein|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|yacO] and [protein|8A2898B80D0FFA56C5AA86C6F09D886A62AABBDA|rlmP], according to UniProt)
Structure
[PDB|4JAK] (from ''E. coli'', 45% identity, 72% similarity) [Pubmed|23804755]
[AF|O31590]
Expression and Regulation
Operons
genes
[gene|506BD8F55C235CCCF5045073AEC93D8B1E4FB8DF|yhbB]-[gene|03BA7AEE17739689D632A35D9C3329EA0982C98A|cspR]
description
[Pubmed|12662922]
regulation
''[wiki|yhbB]-[wiki|cspR]'': expressed in the mother cell early during [wiki|sporulation] ([wiki|SigE]) [Pubmed|12662922]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|12662922], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
genes
[gene|03BA7AEE17739689D632A35D9C3329EA0982C98A|cspR]
description
[Pubmed|22383849]
regulation
''[wiki|yhbB]-[wiki|cspR]'': expressed in the mother cell early during [wiki|sporulation] ([wiki|SigE]) [Pubmed|12662922]
Biological materials
Mutant
BKE08930 ([gene|03BA7AEE17739689D632A35D9C3329EA0982C98A|cspR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTCTGTTTTTCTTATCC, downstream forward: _UP4_TAGTGAAAAACCCGCTCATC
BKK08930 ([gene|03BA7AEE17739689D632A35D9C3329EA0982C98A|cspR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTCTGTTTTTCTTATCC, downstream forward: _UP4_TAGTGAAAAACCCGCTCATC
References
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Time of last update: 2024-10-02 05:57:43
Author of last update: Jstuelk