tgt

tgt
168

tRNA-guanine transglycosylase

locus
BSU_27710
Molecular weight
43.55 kDa
pI
6.15
Protein length
Gene length
function
tRNA modification
product
tRNA-guanine transglycosylase
essential
no
ec
2.4.2.29
synonyms
tgt

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0343 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,832,727  2,833,872
The protein
Catalyzed reaction/ biological activity
7-aminomethyl-7-carbaguanine + guanosine34 in tRNA --> 7-aminomethyl-7-carbaguanosine34 in tRNA + guanine (according to UniProt)
Protein family
queuine tRNA-ribosyltransferase family (single member, according to UniProt)
Structure
[PDB|1PUD] (from Zymomonas mobilis, 52% identity) [pubmed|8654383]
[AF|O32053]
[wiki|Localization]
cytosol(according to UniProt)
Expression and Regulation
Operons
genes
[gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]-[gene|6CDF1836F5B613A6718308A4A27D713193EA197B|yrzS]-[gene|23174B2A19DEDF2F20858A005D0681D36E15E6E3|queA]-[gene|0383C8214ADE2FF70CFDB953A65648B37A9D3216|tgt]-[gene|D15B74263167DAD3FA9B7384B5AA4897EAD498F2|yrbF]
description
[pubmed|22383849]
regulation
the mRNA is processed between [gene|0383C8214ADE2FF70CFDB953A65648B37A9D3216|tgt] and [gene|D15B74263167DAD3FA9B7384B5AA4897EAD498F2|yrbF] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
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[gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]→[gene|D15B74263167DAD3FA9B7384B5AA4897EAD498F2|yrbF]

2024-10-10 03:53:21

Jstuelk

98

b67b31b8062f65eb2771aa03f56aa1b45473760c

1495BBE3D6ACD7DA206B13AA8930F5C46B2B7309

Biological materials
Mutant
BKE27710 ([gene|0383C8214ADE2FF70CFDB953A65648B37A9D3216|tgt]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27710 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTTTGATGAGGCCTCCT,  downstream forward: _UP4_TAAATAAACATAACAGGCTT
BKK27710 ([gene|0383C8214ADE2FF70CFDB953A65648B37A9D3216|tgt]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27710 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTTTGATGAGGCCTCCT,  downstream forward: _UP4_TAAATAAACATAACAGGCTT
References
36815589,6804468,14523925,12949492,8494901,7698334,7893665,7615526,8608154,12877882,8654383,29794222

0383C8214ADE2FF70CFDB953A65648B37A9D3216

Page visits: 2766

Time of last update: 2024-10-12 18:00:34

Author of last update: Jstuelk