vmlR
168
F-type ATP-binding cassette protein, allosterically dissociates antibiotics (virginiamycin M, lincomycin) from their ribosomal binding sites
locus
BSU_05610
Molecular weight
62.57 kDa
pI
6.73
function
dissociation of antibiotics (virginiamycin M, lincomycin) from the ribosome
product
ABCF ATPase
essential
no
synonyms
vmlR, expZ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0488 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
604,736 606,379
The protein
Protein family
[wiki|ABC transporter superfamily] (according to UniProt)
[wiki|ABCF ATPase subfamily] [pubmed|30597160]
[wiki|Domains]
2 [wiki|ABC transporter domain]s (aa 5-200, aa 292-504) (according to UniProt)
Structure
[PDB|6HA8] [pubmed|30126986]
[AF|P39115]
[wiki|Localization]
cytoplasm [pubmed|30597160]
Expression and Regulation
Operons
genes
[gene|032BF9280D006DD6D7C7D07A983C95E1433AC88C|vmlR]
description
[Pubmed|16109936]
regulation
induced by virginiamycin M or lincomycin due to antibiotic-induced ribosome stalling during translation of an upstream open reading frame in the [gene|032BF9280D006DD6D7C7D07A983C95E1433AC88C|vmlR] leader region resulting in transcription anti termination [Pubmed|35699226,16109936]
[protein|43CFA3477B4EEB4CE0B2DF63ED2B5EDB171E79C7|nusG]-dependent RNA polymerase pausing in the [gene|032BF9280D006DD6D7C7D07A983C95E1433AC88C|vmlR] leader prevents leaky expression in the absence of antibiotic [pubmed|35699226]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16109936], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
A [protein|search|ncRNA] is predicted between '[protein|032BF9280D006DD6D7C7D07A983C95E1433AC88C|vmlR]' and '[protein|C7FEA1D9C7C96C39CEE83CE3F13D412DE6AFE10E|ydgF]' [PubMed|20525796]
Biological materials
Mutant
MGNA-C159 (expZ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2157 NBRP B. subtilis, Japan]
BKE05610 ([gene|032BF9280D006DD6D7C7D07A983C95E1433AC88C|vmlR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCATATCCCTCGCTTTA, downstream forward: _UP4_TGAGGGAGAAAGTTCAAGCG
BKK05610 ([gene|032BF9280D006DD6D7C7D07A983C95E1433AC88C|vmlR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCATATCCCTCGCTTTA, downstream forward: _UP4_TGAGGGAGAAAGTTCAAGCG
References
Reviews
Original Publications
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Time of last update: 2024-12-31 00:02:38
Author of last update: Jstuelk