lytS

lytS
168

two-component sensor kinase, control of pyruvate utilization

locus
BSU_28930
Molecular weight
64.68 kDa
pI
8.24
Protein length
Gene length
function
control of pyruvate utilization
product
two-component sensor kinase
essential
no
synonyms
lytS

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3275 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,956,486  2,958,267
Phenotypes of a mutant
no growth with pyruvate as the single carbon source [Pubmed|27422364]
The protein
Catalyzed reaction/ biological activity
ATP + [protein|search|L-histidine] --> ADP + [protein|search|N-phospho-L-histidine] (according to UniProt)
[wiki|Domains]
5 transmembrane regions
[wiki|GAF domain] (aa 238-363) (according to UniProt)
[wiki|Histidine kinase domain] (aa 363-580) (according to UniProt)
Structure
[AF|P94513]
Modification
autophosphorylation on a His residue
[wiki|Localization]
membrane (putative, due to presence of 5 membrane-spanning domains, according to Uniprot)
Expression and Regulation
Operons
genes
[gene|02FAD7D3A73CC0462D0116F4E5BA9505CBF7FCDD|lytS]-[gene|53BA24E91EEC797C95B8975C487F3C50CBBFA2E3|lytT]
description
[Pubmed|22383849]
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[gene|02FAD7D3A73CC0462D0116F4E5BA9505CBF7FCDD|lytS]→[gene|53BA24E91EEC797C95B8975C487F3C50CBBFA2E3|lytT]

2024-09-20 03:59:47

Jstuelk

73

b3efd5a555d16204bd59903e25a242e8ddfecdb4

7F602BF3F8F799BD20F08F804BC1A557D704AB63

Biological materials
Mutant
MGNA-A015 (lytS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/15 NBRP B. subtilis, Japan]
BKE28930 ([gene|02FAD7D3A73CC0462D0116F4E5BA9505CBF7FCDD|lytS]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28930 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCATGTCACCTGTTCTT,  downstream forward: _UP4_GAACATGCTCAGGGTGTTAA
BKK28930 ([gene|02FAD7D3A73CC0462D0116F4E5BA9505CBF7FCDD|lytS]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28930 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCATGTCACCTGTTCTT,  downstream forward: _UP4_GAACATGCTCAGGGTGTTAA
References
Reviews
29208748,29354650
Original Publications
10094672,27422364,28974613

02FAD7D3A73CC0462D0116F4E5BA9505CBF7FCDD

Page visits: 2558

Time of last update: 2024-10-15 19:18:48

Author of last update: Melvin.boenninger