xpt
168
xanthine phosphoribosyltransferase
locus
BSU_22070
Molecular weight
20.89 kDa
pI
5.84
function
purine salvage and interconversion
product
xanthine phosphoribosyltransferase
essential
no
ec
2.4.2.22
synonyms
xpt
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0503 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,319,440 → 2,320,024
The protein
Catalyzed reaction/ biological activity
5-phospho-α-D-ribose 1-diphosphate + xanthine --> diphosphate + XMP (according to UniProt)
Protein family
[wiki|Purine/pyrimidine phosphoribosyltransferase family] (according to UniProt)
Structure
[PDB|1Y0B], [PDB|2FXV] (complex with GMP), [PDB|1Y27] (the riboswitch in complex with guanine)
[AF|P42085]
Effectors of protein activity
strongly inhibited by pGpp and ppGpp [pubmed|32446804]
weakly inhibited by pppGpp [pubmed|32446804]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
Expression and Regulation
Operons
genes
[gene|02C84C9A8C0008CC253CBF13584A4B5B2D2B510C|xpt]-[gene|7D56F85FBD3AAC66607DD7465592EEAE09D61C98|pbuX]
description
[Pubmed|9098051]
regulation
induced in the absence of guanine ([wiki|G-box]) [Pubmed|12787499]
regulatory mechanism
G-box: RNA switch, [wiki|riboswitch] [Pubmed|9098051,12787499], in [regulon|other_regulator:G-box|G-box]
[protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, (molecular inducer: PRPP) [Pubmed|11591660], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9098051], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A870 (xpt::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/870 NBRP B. subtilis, Japan]
BKE22070 ([gene|02C84C9A8C0008CC253CBF13584A4B5B2D2B510C|xpt]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTGTCTACCTCCGTTA, downstream forward: _UP4_TCCTTCGTACAGGAGGTTCA
BKK22070 ([gene|02C84C9A8C0008CC253CBF13584A4B5B2D2B510C|xpt]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTGTCTACCTCCGTTA, downstream forward: _UP4_TCCTTCGTACAGGAGGTTCA
lacZ fusion
pGP430 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2025-04-08 11:36:33
Author of last update: Jstuelk