odhB
168
2-oxoglutarate dehydrogenase complex (dihydrolipoamide transsuccinylase, E2 subunit)
locus
BSU_19360
Molecular weight
45.83 kDa
pI
4.86
function
TCA cycle
product
2-oxoglutarate dehydrogenase complex
essential
no
ec
2.3.1.61
synonyms
odhB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0508 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,107,505 2,108,758
The protein
Catalyzed reaction/ biological activity
(R)-N6-dihydrolipoyl-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + succinyl-CoA --> (R)-N6-(S8-succinyldihydrolipoyl)-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + CoA (according to UniProt)
Protein family
[wiki|2-oxoacid dehydrogenase family] (according to UniProt)
[wiki|Domains]
[wiki|Lipoyl-binding domain] (aa 1-76) (according to UniProt)
[wiki|Peripheral subunit-binding domain] (PSBD) (aa 123-160) (according to UniProt)
[wiki|Cofactors]
lipoic acid (on Lys-42), can be removed by [protein|A0A6CB19191A251BB5600C18E039978A9A34933C|srtN] [pubmed|28900027]
Structure
[PDB|3DUF] (EI of the PDH complex from ''Geobacillus stearothermophilus'', 38% identity) [Pubmed|19081062]
[AF|P16263]
Modification
phosphorylated on several Arg residues [Pubmed|24263382]
Paralogous protein(s)
[protein|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB], [protein|2F40086E35FA32136B9A89C530A86D714FE9460C|pdhC], [protein|C4B6C3EE560C8BD353FEABB1A607C89C20DC8D34|acoC]
[wiki|Localization]
membrane associated [Pubmed|18763711]
Additional information
extensive information on the structure and enzymatic properties of 2-oxoglutarate dehydrogenase can be found at [http://www.proteopedia.org/wiki/index.php/2-Oxoglutarate_Dehydrogenase Proteopedia]
Expression and Regulation
Operons
genes
[gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA]-[gene|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB]
description
[Pubmed|11976317]
regulation
repressed by glucose (2.4-fold) ([protein|search|CcpA]) [Pubmed|12850135]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|12850135], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1508153], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP4471 (trpC2 Δ[gene|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB]::neo), available in [wiki|Jörg Stülke]'s lab
GP1276 Δ(''[gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA]-[gene|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB])''::''cat'', available in [wiki|Jörg Stülke]'s lab [Pubmed|24178028]
GP2332 Δ([gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA]-[gene|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB])::''kan'', Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'', available in [wiki|Jörg Stülke]'s lab
GP2334 Δ([gene|3B54AD8514709655BA829347B7BD5B38EF7C08ED|odhA]-[gene|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB])::''lox72'', Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'', available in [wiki|Jörg Stülke]'s lab
BKE19360 ([gene|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCCGCCATTTTTTCATTTC, downstream forward: _UP4_TAATAAAAAAGGGTACATCA
BKK19360 ([gene|02BA02D10DFB06E51101D8CF76BCF5BED94D7CA2|odhB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCCGCCATTTTTTCATTTC, downstream forward: _UP4_TAATAAAAAAGGGTACATCA
Expression vectors
pGP1145 (N-terminal Strep-tag, for [wiki|SPINE], purification from ''B. subtilis'', in [wiki|pGP380]) (available in [wiki|Stülke] lab) [Pubmed|20933603]
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Stülke] lab [Pubmed|20933603]
FLAG-tag construct
GP1424 (spc, based on [wiki|pGP1331]), available in the [wiki|Stülke] lab
References
Reviews
Original Publications
Page visits: 6027
Time of last update: 2024-12-21 14:29:31
Author of last update: Robert.warneke