pdeH
168
c-di-GMP degrading phosphodiesterase
locus
BSU_31740
Molecular weight
47.76 kDa
pI
5.36
function
degradation of c-di-GMP
product
c-di-GMP degrading phosphodiesterase
essential
no
ec
3.1.3.71
synonyms
pdeH, yuxH, comB, yufA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3434 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,258,037 3,259,266
Phenotypes of a mutant
about three-fold increase of intracellular levels of c-di-GMP [pubmed|31138629]
defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827]
additional information
the [gene|02628F274952F9AEEE9F2224B771A4CE4AAD1C8D|pdeH] gene is often inactivated during adaptation to plants [pubmed|37466402]
The protein
Catalyzed reaction/ biological activity
degradation of c-di-GMP [Pubmed|23893111]
[wiki|Domains]
contains an EAL domain [Pubmed|22821967]
EAL domain (aa 1-209) (according to UniProt)
HDOD domain (aa 203-392) (according to UniProt)
Structure
[AF|P14203]
Expression and Regulation
Operons
genes
[gene|02628F274952F9AEEE9F2224B771A4CE4AAD1C8D|pdeH]
description
[Pubmed|2507523]
regulation
repressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647,15687200]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|14651647,15687200], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2507523], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A606 (yufA/yuxH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/606 NBRP B. subtilis, Japan]
GP1590 (kan) available in [wiki|Jörg Stülke]'s lab
BKE31740 ([gene|02628F274952F9AEEE9F2224B771A4CE4AAD1C8D|pdeH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGATTTATCCCCCTTAC, downstream forward: _UP4_TATTTAGAGGCTCTGGAATG
BKK31740 ([gene|02628F274952F9AEEE9F2224B771A4CE4AAD1C8D|pdeH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGATTTATCCCCCTTAC, downstream forward: _UP4_TATTTAGAGGCTCTGGAATG
References
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Time of last update: 2024-12-22 01:29:07
Author of last update: Jstuelk