pfeT
168
Fe2+ efflux pump, P1B4-type ATPase, protects the cell against iron intoxication
locus
BSU_13850
Molecular weight
68.39 kDa
pI
4.93
function
protection against toxic iron
product
Fe2+ efflux pump
essential
no
ec
3.6.3.5
synonyms
pfeT, ykvW, zosA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2217 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,451,371 1,453,284
Phenotypes of a mutant
sensitivity to iron, this can be suppressed by low levels of Mn2+ [Pubmed|26261021]
reduced genetic competence, can be rescued by the addition of excess zinc [Pubmed|21813502]
reduced expression of [gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK], control is exerted at the post-transcriptional level and can be rescued by the addition of excess zinc [Pubmed|21813502]
The protein
Catalyzed reaction/ biological activity
ATP + H2O + Zn2+ --> ADP + H+ + phosphate + Zn2+ (according to UniProt)
P-type zinc-transporting ATPase [Pubmed|12180919]
Protein family
[wiki|cation transport ATPase (P-type) (TC 3.A.3) family] (according to UniProt)
Structure
[PDB|4UMV] (zinc transporter from Shigella sonnei, 39% identity) [pubmed|25132545]
[AF|O31688]
Paralogous protein(s)
[protein|5BC6B02770E74FF6D453742832574A87BB2369B8|cadA], [protein|727024F7B1AC19676ED4B516CF46A47B1328310B|copA]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|017A57F27DD8E515242FB658A61E74C1D58273CC|pfeT]
description
[Pubmed|12180919]
regulation
induced by hydrogen peroxide ([protein|D0982500E52577D52FADF775C0E512A4B9657B79|ahpC], [protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR]) [Pubmed|12180919]
induced by iron excess ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]) [pubmed|22194458]
regulatory mechanism
[protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR]: repression, [Pubmed|31988078,22194458,12180919], in [regulon|protein:00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR regulon]
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: activation, [pubmed|31988078], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12180919], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
A [protein|search|ncRNA] is predicted between '[protein|0B299F9459023306FA91298A6162A09E4A87C3B2|stoA]' and '[protein|017A57F27DD8E515242FB658A61E74C1D58273CC|pfeT]' [PubMed|20525796]
Biological materials
Mutant
MGNA-B329 (ykvW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1328 NBRP B. subtilis, Japan]
GP3357 ([gene|017A57F27DD8E515242FB658A61E74C1D58273CC|pfeT]::phleo trpC2) available in [wiki|Jörg Stülke]'s lab
BKE13850 ([gene|017A57F27DD8E515242FB658A61E74C1D58273CC|pfeT]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCGAATTCTCCTCTCT, downstream forward: _UP4_TAAAACCAGCAGCCTAATCA
BKK13850 ([gene|017A57F27DD8E515242FB658A61E74C1D58273CC|pfeT]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCGAATTCTCCTCTCT, downstream forward: _UP4_TAAAACCAGCAGCCTAATCA
References
Reviews
Original Publications
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Time of last update: 2024-10-14 07:36:04
Author of last update: LorenzDemann