spoIIIAH
168
component of the [protein|search|SpoIIIA]-[protein|3282D2C25468776881778006F182FCF322C4821D|spoIIQ] type III secretion system residing in the forespore membrane, required for [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG] activation
locus
BSU_24360
Molecular weight
23.58 kDa
pI
4.63
function
activation of [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG], forespore encasement by the spore coat
product
part of the transmembrane channel linking the mother cell and the forespore
essential
no
synonyms
spoIIIAH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5829 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,532,353 2,533,009
Phenotypes of a mutant
reduced sporulation efficiency (1 to 10% compared to wild type) [Pubmed|26735940]
the ''[gene|8F3751A3D0910C9BED7FBCE90AFF7FDACFDCBC0B|spoIIIL] [gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]'' double mutant has a severe sporulation defect (0.001%) [Pubmed|26735940]
block of sporulation after engulfment
The protein
Catalyzed reaction/ biological activity
required for forespore encasement by the spore coat [Pubmed|22171814]
[wiki|Domains]
contains a ring-building motif (RBM) domain [pubmed|34808342]
Structure
[PDB|3UZO]; [PDB|3TUF] (the [protein|3282D2C25468776881778006F182FCF322C4821D|spoIIQ]-[protein|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH] pore forming complex) [Pubmed|22431604,22431613]
[AF|P49785]
[wiki|Localization]
membrane protein, forms a transmembrane channel linking the mother cell and the forespore (with [protein|3282D2C25468776881778006F182FCF322C4821D|spoIIQ]) [Pubmed|22431604,22431613,22171814,18485064]
proper recruitment to the sporulation septum on the mother cell side requires [protein|3282D2C25468776881778006F182FCF322C4821D|spoIIQ] [Pubmed|23834622]
Expression and Regulation
Operons
genes
[gene|70408C17CF8681998F7FD933D4BFF71D052626D9|yqhV]-[gene|5FE74F270B28A404BFE0DAA4EB243009EEDEACF0|spoIIIAA]-[gene|D20198968665D3726839B2DC7F0638614319B853|spoIIIAB]-[gene|5CDBE6E299C7E6606D3C52E509CFED6F41F1E3F8|spoIIIAC]-[gene|576E36DA79F1ED097D649C35C5B59622FF1CFEF3|spoIIIAD]-[gene|9D4AE9AE191BFA82791549852782DC1B959A6897|spoIIIAE]-[gene|8A8C67AB955660FA41039B45F60A5B9F7B57704E|spoIIIAF]-[gene|5456B0C27EAE89F5B9E750D65E1E3C6EDA27C9FA|spoIIIAG]-[gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]
description
[Pubmed|1766372]
regulation
both promoters: expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|18485064], switched off by [wiki|SpoIIID] [Pubmed|15383836]
regulatory mechanism
[protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,18485064], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
genes
[gene|5456B0C27EAE89F5B9E750D65E1E3C6EDA27C9FA|spoIIIAG]-[gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]
description
[Pubmed|17693505]
regulation
both promoters: expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|18485064], switched off by [wiki|SpoIIID] [Pubmed|15383836]
additional information
for a discussion of the relevance of the operon-internal promoter, see [pubmed|35913150]
genes
[gene|5FE74F270B28A404BFE0DAA4EB243009EEDEACF0|spoIIIAA]-[gene|D20198968665D3726839B2DC7F0638614319B853|spoIIIAB]-[gene|5CDBE6E299C7E6606D3C52E509CFED6F41F1E3F8|spoIIIAC]-[gene|576E36DA79F1ED097D649C35C5B59622FF1CFEF3|spoIIIAD]-[gene|9D4AE9AE191BFA82791549852782DC1B959A6897|spoIIIAE]-[gene|8A8C67AB955660FA41039B45F60A5B9F7B57704E|spoIIIAF]-[gene|5456B0C27EAE89F5B9E750D65E1E3C6EDA27C9FA|spoIIIAG]-[gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]
description
[Pubmed|18485064]
regulation
both promoters: expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|18485064], switched off by [wiki|SpoIIID] [Pubmed|15383836]
Biological materials
Mutant
BKE24360 ([gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]::erm trpC2)available at [http://bgsc.org/getdetail.php?bgscid=BKE24360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCAAACGGTTTGTTTTTTAA, downstream forward: _UP4_TAAGAATGAGGGAAAAAAGC
BKK24360 ([gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCAAACGGTTTGTTTTTTAA, downstream forward: _UP4_TAAGAATGAGGGAAAAAAGC
labs
[wiki|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-20 16:47:06
Author of last update: Jstuelk