perR
168
transcriptional repressor of the peroxide regulon, sensor of the intracellular Fe/Mn ratio
locus
BSU_08730
Molecular weight
16.28 kDa
pI
5.89
function
regulation of the response to peroxide
product
transcriptional repressor ([wiki|Fur family])
essential
no
synonyms
perR, ygaG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0735 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
944,487 944,924
Phenotypes of a mutant
resistant to hydrogen peroxide, accumulates a porphyrin-like compound, and grows very slowly (due to heme sequestration by [protein|B0A1AB6E920E6CBEE45115297599AF9A80972E38|katA]) [Pubmed|22194458]
The protein
Protein family
[wiki|Fur family] (according to UniProt)
[wiki|Cofactors]
contains an Fe2+ at the regulatory site and Zn2+ [Pubmed|22797754]
Structure
[PDB|2RGV] [PDB|3F8N]
[AF|P71086]
Modification
selective metal catalyzed oxidation of two histidine residues of the regulatory site results in induction (loss of DNA-binding activity) [Pubmed|19508285]
Effectors of protein activity
responds to the presence of hydrogen peroxide [pubmed|29738773]
Paralogous protein(s)
[protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur], [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR]
description
[Pubmed|12029044]
regulation
induced in the presence of hydrogen peroxide ([protein|search|PerR]) [Pubmed|12029044]
regulatory mechanism
[protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR]: repression, [Pubmed|12029044], in [regulon|protein:00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12029044], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|3C139835154FA236066CF2FB82A3C32E3A72D65E|ygaF]-[gene|00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR]
description
[Pubmed|22383849]
Biological materials
Mutant
GP3171 [gene|00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR]::kan trpC2 available in [wiki|Jörg Stülke]'s lab
MGNA-A258 (ygaG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/258 NBRP B. subtilis, Japan]
HB0509 (spc), available in [wiki|John Helmann]'s and [wiki|Jörg Stülke]'s labs, also GP868 (''fur::mls'', ''perR::spc'').
1A903 ( ''perR''::''kan''), [Pubmed|12029044], available at the [http://bgsc.org/getdetail.php?bgscid=1A903 Bacillus Genetic Stock Center]
BKE08730 ([gene|D0982500E52577D52FADF775C0E512A4B9657B79|ahpC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGTCATGCACCTCTCT, downstream forward: _UP4_TAAAAATAAGCTGACCGCAC
BKK08730 ([gene|D0982500E52577D52FADF775C0E512A4B9657B79|ahpC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGTCATGCACCTCTCT, downstream forward: _UP4_TAAAAATAAGCTGACCGCAC
labs
[wiki|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-21 21:56:29
Author of last update: Jstuelk