gmuB
168
glucomannan-specific permease of the [wiki|phosphotransferase systems|phosphotransferase system], EIIB of the [category|SW.1.2.2|PTS]
locus
BSU_05810
Molecular weight
11.30 kDa
pI
4.72
function
glucomannan uptake and phosphorylation
product
glucomannan-specific lichenan-specific [category|SW.1.2.2|PTS], EIIB component
essential
no
synonyms
gmuB, ydhM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1440 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
626,622 626,933
The protein
Catalyzed reaction/ biological activity
D-cellobiose + Nπ-phospho-L-histidyl-[protein] --> 6-phospho-β-D-glucosyl-(1→4)-D-glucose + L-histidyl-[protein] (according to UniProt)
Protein family
[category|SW.1.2.2|PTS] permease, lactose family [Pubmed|10627040]
[wiki|Domains]
[wiki|PTS EIIB domain] type-3 (aa 1-103) (according to UniProt)
Structure
[PDB|4MGE] (from B. anthracis, 46% identity)
[AF|O05505]
Paralogous protein(s)
[protein|65FC7F4ED92F747DB7BC5672DCA2D2320A471134|licB]
Expression and Regulation
Operons
genes
[gene|00A4A90685CED4AD46829C45718CF26F1D62F5D3|gmuB]-[gene|44014896157718D3A6190DFDBD663C5CB9DA63FC|gmuA]-[gene|76324E0A6CAA8E1DD0B0C6FAE1CAB402231BF7CC|gmuC]-[gene|D8C81A8768E9C6B47B5E196B54DC2A3A8256E7E6|gmuD]-[gene|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|gmuR]-[gene|EB6DE119E6FB18413DB2C2B62B114FB787164F74|gmuE]-[gene|35225776F6044513D3E77DBBD6A7A8FE976F506A|gmuF]-[gene|289ACC9D13BBFAA5C083EE525ECCC32DB06E075F|gmuG]
description
[Pubmed|18177310]
regulation
induced by cellobiose ([protein|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|gmuR]) [Pubmed|18177310]
regulatory mechanism
[protein|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|gmuR]: repression, [Pubmed|18177310], in [regulon|protein:64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|gmuR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|18177310], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: activation, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|18177310], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-C188 (ydhM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2186 NBRP B. subtilis, Japan]
BKE05810 ([gene|00A4A90685CED4AD46829C45718CF26F1D62F5D3|gmuB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTGCTATCCCCCCTGTT, downstream forward: _UP4_TTGTCCTTAATGGTGAATCA
BKK05810 ([gene|00A4A90685CED4AD46829C45718CF26F1D62F5D3|gmuB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTGCTATCCCCCCTGTT, downstream forward: _UP4_TTGTCCTTAATGGTGAATCA
References
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Time of last update: 2024-12-30 08:06:18
Author of last update: Melvin.boenninger