FlhG

• Description: GTPase activating protein, activates FlhF, activates assembly of the flagellar C ring, control of flagellar basal body position
  
  

Gene name	flhG
Synonyms	ylxH
Essential	no
Product	GTPase activating protein
Function	activation of FlhF
Gene expression levels in SubtiExpress: flhG
Interactions involving this protein in SubtInteract: FlhG
MW, pI	33 kDa, 9.648
Gene length, protein length	894 bp, 298 aa
Immediate neighbours	flhF, cheB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis, resistance against toxins/ antibiotics

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16410

Phenotypes of a mutant

• susceptible to acriflavine and ethidium bromide, and severe growth inhibition as surfactin concentration increased up to 100ug/ml.

Database entries

• BsubCyc: BSU16410

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • binds FlhF and stimulates its GTPase activity PubMed
  • controls the distance between the flagellar bodies PubMed

• Protein family: View classification (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • forms homodimers PubMed
  • FlhG-FlhF (FlhG binds the GTP-bound homodimer of FlhF) PubMed
  • FlhG-(FliY-FliM)-FliG PubMed

• Localization: cytoplasm (homogeneous) and flagellar basal body PubMed

Database entries

• BsubCyc: BSU16410

• Structure:
  • 4RZ3 (from Geobacillus thermodenitrificans) PubMed
  • 3SYN (complex with FlhF) PubMed

• UniProt:
  • P40742
  • 3SYN (complex with FlhF) PubMed

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: part of the fla-che operon
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: ylxH PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed

• Regulation: see fla-che operon

• Regulatory mechanism: see fla-che operon

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

<pubmed> 26195616,, </pubmed>

Original Publications

<pubmed>15066026,17850253,14651647, 16479537 9657996,8157612,15175317 22056770 23190039 24386445 25733861</pubmed>