Mbl

• Description: cell shape-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex, required for CwlO activity
  
  

Gene name	mbl
Synonyms
Essential	no
Product	MreB-like protein
Function	cell shape determination
Gene expression levels in SubtiExpress: mbl
Interactions involving this protein in SubtInteract: Mbl
MW, pI	35 kDa, 5.669
Gene length, protein length	999 bp, 333 aa
Immediate neighbours	flhO, spoIIID
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell shape, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon, stringent response

The gene

Basic information

• Locus tag: BSU36410

Phenotypes of a mutant

• non-viable in the presence of low Mg(2+)
• readily accumulate rsgI suppressor mutants PubMed

Database entries

• BsubCyc: BSU36410

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • forms helical filaments in a heterologous system PubMed
  • required for CwlO activity PubMed

• Protein family: ftsA/mreB family (according to Swiss-Prot)

• Paralogous protein(s): MreB, MreBH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • part of the cell wall biosynthetic complex PubMed
  • MreB-Mbl PubMed
  • Mbl-MreBH PubMed
  • RodZ-Mbl PubMed

• Localization:
  • during logarithmic growth, Mbl forms discrete patches that move processively along peripheral tracks perpendicular to the cell axis PubMed
  • forms transverse bands as cells enter the stationary phase PubMed
  • close to the inner surface of the cytoplasmic membrane PubMed
  • reports on helical structures formed by Mbl PubMed seem to be misinterpretation of data PubMed

Database entries

• BsubCyc: BSU36410

• Structure:

• UniProt: P39751

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: usd-spoIIID-mbl-flhO-flhP PubMed

• Expression browser: mbl PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE) PubMed
  • RelA dependent downregulation (Class I) during stringent response PubMed
  • expression is strongly repressed in the presence of aminoguanidine (inhibitor of nitric oxide synthase) PubMed

• Regulatory mechanism:

• Additional information:
  • An antisense RNA is predicted for mbl PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab

• Antibody: available in the Jeff Errington and Peter Graumann labs

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage

References

Localization

<pubmed> 17064365, 20566861 21636744,21636745 22069484</pubmed>

Other original publications

<pubmed>19643765,16832063,11290328,12809607,16101995,12530960,7836311,,16950129 9023218, 19659933 19114499, 21091501 20525796 22048160 22343529 23879732 23869552</pubmed>