SrfAB

• Description: surfactin synthetase / competence
  
  

Gene name	srfAB
Synonyms	comL
Essential	no
Product	surfactin synthetase / competence
Function	antibiotic synthesis
Gene expression levels in SubtiExpress: srfAB
MW, pI	400 kDa, 4.903
Gene length, protein length	10761 bp, 3587 aa
Immediate neighbours	srfAA, comS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds, membrane proteins, phosphoproteins

This gene is a member of the following regulons

Abh regulon, CodY regulon, ComA regulon, PerR regulon, Spx regulon

The gene

Basic information

• Locus tag: BSU03490

Database entries

• BsubCyc: BSU03490

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylation on Ser-999 AND Ser-2045 PubMed
  • phosphorylated on Arg-1378 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: membrane PubMed

Database entries

• BsubCyc: BSU03490

• Structure:

• UniProt: Q04747

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: srfAA-srfAB-comS-srfAC-srfAD PubMed

• Expression browser: srfAB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • repressed during hydrogen peroxide stress (PerR) PubMed
  • expressed at high cell density (ComA) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • ComA: transcription activation PubMed
  • PerR: transcription activation PubMed
  • Spx: transcription repression PubMed
  • Abh: transcription repressionPubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

<pubmed>20735481 </pubmed>

Original publications

<pubmed>17227471,16166527,8288534,10960106, 12642660,8830686,18763711 17218307, 17190806 1715856 16091051 22517742 8355610 22511326, 20817675 </pubmed>