Methods

Here you can find a list of methods for working with Bacillus subtilis:

• Bacterial Two Hybrid: BACTH

• SPINE: A method to detect in vivo protein-protein interactions

• introduction of markerfree deletions: PubMed

• generation of markerfree mutations: PubMed and PubMed

• Mechanical cell disruption and extraction of RNA from B. subtilis: Media:SOP-RNA.pdf

• transformation of wild-type B. subtilis strains incl. NCIB3610: PubMed

• Standard operation procedures (SOPs) of the SYSMO-BACELL consortium

• in vivo detection of protein-protein interactions using DivIVA and GFP PubMed

• ChAP-chip: A modified ChIP-chip protocol for the in vivo identification of binding sites of DNA-binding proteins PubMed

• Live cell imaging of B. subtilis cells using automated time-lapse microscopy PubMed

• mariner transposon mutagenesis for random inducible-expression insertions and transcriptional reporter fusion insertions PubMed

Key references

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