Revision as of 11:49, 11 June 2009

Description: oligopeptide ABC transporter (ATP-binding protein)

Gene name	appF
Synonyms
Essential	no
Product	oligopeptide ABC transporter (ATP-binding protein))
Function	uptake of oligopeptides
MW, pI	36 kDa, 9.645
Gene length, protein length	987 bp, 329 aa
Immediate neighbours	appD, appA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

Locus tag: BSU11370

Phenotypes of a mutant

Database entries

DBTBS entry: no entry

SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

Catalyzed reaction/ biological activity:

Protein family: ABC transporter domain (according to Swiss-Prot)

Paralogous protein(s):

Extended information on the protein

Kinetic information:

Domains:

Modification:

Cofactor(s):

Effectors of protein activity:

Interactions:

Localization: cell membrane (according to Swiss-Prot)

Database entries

Structure:

Swiss prot entry: P42065

KEGG entry: [2]

E.C. number:

Additional information

Expression and regulation

Operon: appD-appF-appA-appB-appC

Sigma factor:

Regulation: repressed by CodY PubMed
- repressed during growth in the presence of branched chain amino acids (CodY) PubMed

Regulatory mechanism: CodY: transcription repression PubMed
- CodY: transcription repression PubMed
- CodY: transcription repression PubMed

Additional information:

Biological materials

Mutant:

Expression vector:

lacZ fusion:

GFP fusion:

two-hybrid system:

Antibody:

Labs working on this gene/protein

Your additional remarks

References

Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed

@@ Line 1: / Line 1: @@
-#REDIRECT[[appA/1]]
+* '''Description:''' oligopeptide ABC transporter (ATP-binding protein) <br/><br/>
+{| align="right" border="1" cellpadding="2"
+|-
+|style="background:#ABCDEF;" align="center"|'''Gene name'''
+|''appF''
+|-
+|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+|-
+|style="background:#ABCDEF;" align="center"| '''Essential''' || no
+|-
+|style="background:#ABCDEF;" align="center"| '''Product''' || oligopeptide ABC transporter (ATP-binding protein))
+|-
+|style="background:#ABCDEF;" align="center"|'''Function''' || uptake of oligopeptides
+|-
+|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 9.645
+|-
+|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 987 bp, 329 aa
+|-
+|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[appD]]'', ''[[appA]]''
+|-
+|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12994&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+|-
+|-
+|-
+|colspan="2" | '''Genetic context''' <br/> [[Image:appF_context.gif]]
+ <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
+|-
+|}
+__TOC__
+<br/><br/>
+=The gene=
+=== Basic information ===
+* '''Locus tag:''' BSU11370
+===Phenotypes of a mutant ===
+=== Database entries ===
+* '''DBTBS entry:''' no entry
+* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11086]
+=== Additional information===
+=The protein=
+=== Basic information/ Evolution ===
+* '''Catalyzed reaction/ biological activity:'''
+* '''Protein family:''' ABC transporter domain (according to Swiss-Prot)
+* '''Paralogous protein(s):'''
+=== Extended information on the protein ===
+* '''Kinetic information:'''
+* '''Domains:'''
+* '''Modification:'''
+* '''Cofactor(s):'''
+* '''Effectors of protein activity:'''
+* '''Interactions:'''
+* '''Localization:''' cell membrane (according to Swiss-Prot)
+=== Database entries ===
+* '''Structure:'''
+* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P42065 P42065]
+* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU11370]
+* '''E.C. number:'''
+=== Additional information===
+=Expression and regulation=
+* '''Operon:''' ''[[appD]]-[[appF]]-[[appA]]-[[appB]]-[[appC]]''
+* '''[[Sigma factor]]:'''
+* '''Regulation:''' repressed by [[CodY]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
+** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
+* '''Regulatory mechanism:''' [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
+** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
+** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed]
+* '''Additional information:'''
+=Biological materials =
+* '''Mutant:'''
+* '''Expression vector:'''
+* '''lacZ fusion:'''
+* '''GFP fusion:'''
+* '''two-hybrid system:'''
+* '''Antibody:'''
+=Labs working on this gene/protein=
+=Your additional remarks=
+=References=
+# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]