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* '''Description:''' sensor kinase of the YvrG-YvrHb two-component system <br/><br/>
* '''Description:''' two-component response regulator <br/><br/>


{| align="right" border="1" cellpadding="2"  
{| align="right" border="1" cellpadding="2"  
|-
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yvrG''
|''yvrHb''
|-
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yvrH ''
|-
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || two-component sensor histidine kinase
|style="background:#ABCDEF;" align="center"| '''Product''' || two-component response regulator
|-
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation cell surface maintenance
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation cell surface maintenance
|-
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 66 kDa, 9.518 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 26.6 kDa, 5.23
|-
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1719 bp, 573 aa
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 714 bp, 237 amino acids
|-
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvrE]]'', ''[[yvrHb]]''
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvrHa]]'', ''[[yvrG]]''
|-
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15311&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/genE_nucleotide.txt    Gene sequence      (+200bp)  ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/genE_protein.txt Protein sequence]'''
|-
|-
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:YvrHa_yvrHb_context.GIF]]
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:yvrG_context.gif]]
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
|-
|-
|}
|}
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=== Basic information ===
=== Basic information ===


* '''Locus tag:''' BSU33210
* '''Locus tag:'''


===Phenotypes of a mutant ===
===Phenotypes of a mutant ===
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* '''DBTBS entry:''' no entry
* '''DBTBS entry:''' no entry


* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14146]
* '''SubtiList entry:''' no entry


=== Additional information===
=== Additional information===


=The protein=
=The protein=
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=== Basic information/ Evolution ===
=== Basic information/ Evolution ===


* '''Catalyzed reaction/ biological activity:''' ATP + protein L-histidine = ADP + protein N-phospho-L-histidine (according to Swiss-Prot) phosphorylation of the [[YvrHb]] response regulator [http://www.ncbi.nlm.nih.gov/sites/entrez/16306698 PubMed]
* '''Catalyzed reaction/ biological activity:''' transcription activator [http://www.ncbi.nlm.nih.gov/sites/entrez/16306698 PubMed]
 
* '''Protein family:''' OmpR family
 
* '''Paralogous protein(s):'''
 
=== The YvrHb regulon ===


* '''Protein family:'''
subject to positive control: ''[[dltA]]-[[dltB]]-[[dltC]]-[[dltD]]-[[dltE]], [[sigX]]-[[rsiX]], [[sunA]], [[sunT]]-[[bdbA]]-[[yolJ]]-[[bdbB]], [[wapA]]-[[yxxG]], [[wprA]], [[yvrI]]-[[yvrHa]]''  [http://www.ncbi.nlm.nih.gov/sites/entrez/16306698 PubMed]


* '''Paralogous protein(s):''' [[CssS]], [[YkoH]], [[KinE]], [[YclK]]
subject to negative control: ''[[lytA]]-[[lytB]]-[[lytC]]''  [http://www.ncbi.nlm.nih.gov/sites/entrez/16306698 PubMed]


=== Extended information on the protein ===
=== Extended information on the protein ===
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* '''Kinetic information:'''
* '''Kinetic information:'''


* '''Domains:'''  two transmembrane segments, C-terminal histidine phosphotransferase domain
* '''Domains:'''   


* '''Modification:'''
* '''Modification:''' phosphorylation (Asp) by [[YvrG]]  [http://www.ncbi.nlm.nih.gov/sites/entrez/16306698 PubMed]


* '''Cofactor(s):'''
* '''Cofactor(s):'''
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* '''Interactions:''' YvrG-[[YvrHb]]  [http://www.ncbi.nlm.nih.gov/sites/entrez/16306698 PubMed]
* '''Interactions:''' YvrG-[[YvrHb]]  [http://www.ncbi.nlm.nih.gov/sites/entrez/16306698 PubMed]


* '''Localization:''' cell membrane (according to Swiss-Prot)
* '''Localization:''' cytoplasm (according to Swiss-Prot)


=== Database entries ===
=== Database entries ===
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* '''Structure:'''
* '''Structure:'''


* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O34989 O34989]
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P94504 P94504]


* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU33210]
* '''KEGG entry:''' no entry


* '''E.C. number:'''
* '''E.C. number:'''
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* '''Operon:'''  
* '''Operon:'''  


* '''Sigma factor:'''  
* '''[[Sigma factor]]:'''  


* '''Regulation:'''  
* '''Regulation:'''  
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* '''Regulatory mechanism:'''  
* '''Regulatory mechanism:'''  


* '''Additional information:'''  
* '''Additional information:'''


=Biological materials =
=Biological materials =

Revision as of 13:52, 8 June 2009

  • Description: two-component response regulator

Gene name yvrHb
Synonyms yvrH
Essential no
Product two-component response regulator
Function regulation cell surface maintenance
MW, pI 26.6 kDa, 5.23
Gene length, protein length 714 bp, 237 amino acids
Immediate neighbours yvrHa, yvrG
Gene sequence (+200bp) Protein sequence
Genetic context



The gene

Basic information

  • Locus tag:

Phenotypes of a mutant

unusual autolysis and higher susceptibility to cell envelope antibiotics (bacitracin, aztreonam, cefepime, fosfomycin) PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: no entry

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transcription activator PubMed
  • Protein family: OmpR family
  • Paralogous protein(s):

The YvrHb regulon

subject to positive control: dltA-dltB-dltC-dltD-dltE, sigX-rsiX, sunA, sunT-bdbA-yolJ-bdbB, wapA-yxxG, wprA, yvrI-yvrHa PubMed

subject to negative control: lytA-lytB-lytC PubMed

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation (Asp) by YvrG PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: no entry
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

<pubmed>16306698, </pubmed>

  1. Serizawa M, Kodama K, Yamamoto H, Kobayashi K, Ogasawara N, Sekiguchi J. (2005) Functional analysis of the YvrGHb two-component system of Bacillus subtilis: identification of the regulated genes by DNA microarray and northern blot analyses. Biosci. Biotechnol. Biochem. 69: 2155-2169. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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