PAC5: Difference between revisions
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[[File:PAC5.jpeg|250px|thumb|right|'''pAC5: click to enlarge''']] | [[File:PAC5.jpeg|250px|thumb|right|'''pAC5: click to enlarge''']] | ||
[[File:PAC5_cloning_region.jpeg|250px|thumb|right|'''pAC5 cloning region: click to enlarge''']] | [[File:PAC5_cloning_region.jpeg|250px|thumb|right|'''pAC5 cloning region: click to enlarge''']] | ||
vector for the construction of translational ''lacZ'' fusions that can be integrated at the ''amyE'' site | vector for the construction of translational ''lacZ'' fusions that can be integrated at the ''amyE'' site | ||
Revision as of 11:32, 16 April 2009
vector for the construction of translational lacZ fusions that can be integrated at the amyE site
does not replicate in B. subtilis, integrates into the amyE site
constructed in the Rapoport lab
in E. coli: ampicillin resistance
in B. subtilis: chloramphenicol resistance
sequencing primer:
- PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
- PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
- Catcheckrev: 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’
Martin-Verstraete I, Débarbouillé M, Klier A, Rapoport G: (1992) Mutagenesis of the Bacillus subtilis ‘-12, -24’ promoter of the levanase operon and evidence for the existence of an upstream activating sequence. J Mol Biol , 226:85-99. PubMed