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| * '''Description:''' flottilin-like protein (in addition to [[FloT]]), resistence protein (against sublancin), accessory role in resistance to cefuroxime<br/><br/>
| | {{#bank: FloA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''floA''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' yqfA''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || flottilin-like protein
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| |style="background:#ABCDEF;" align="center"|'''Function''' || resistance against sublancin
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=yqfA floA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=FloA FloA]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 4.917
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 993 bp, 331 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqfB]]'', ''[[yqeZ]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25380 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25380 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25380 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:yqfA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yqfA_2617449_2618444_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:yqfA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25380]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[membrane dynamics]]}},
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| {{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}},
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| {{SubtiWiki category|[[resistance against toxins/ antibiotics]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[SigW regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU25380
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| ===Phenotypes of a mutant ===
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| ** reduced [[protein secretion]] {{PubMed|23651456}}
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| ** a ''[[floT]] [[floA]]'' double mutant does not induce [[KinC]]-dependent biofilm formation upon addition of surfactin {{PubMed|20713508}}
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| ** a ''[[floT]] [[floA]]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency {{PubMed|22753055}}
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| ** a ''[[floT]] [[floA]]'' double mutant has a [[sporulation]] defect, due to the lack of [[FtsH]] {{PubMed|22882210}}
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| ** a ''[[floA]]'' mutant produces increased amount of pulcherriminic acid {{PubMed|25909364}}
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| ** a ''[[floA]]'' mutant has reduced sensitivity to vancomycin {{PubMed|25909364}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU25380&redirect=T BSU25380]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yqeZ-yqfA-yqfB.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11651]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** controls protease activity of [[FtsH]] {{PubMed|24222488}}
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| * '''Protein family:''' UPF0365 family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** oligomerization depends on the glutamate-alanine repeats {{PubMed|25909364}}
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| ** [[FloA]]-[[FtsH]] {{PubMed|22882210}}
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| ** [[FloT]]-[[FloA]] {{PubMed|23651456}}
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| ** [[FloA]]-[[PhoR]] {{PubMed|25909364}}
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| * '''[[Localization]]:'''
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| ** membrane [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
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| ** co-localizes with [[FloT]] in discrete foci in the membrane {{PubMed|20713508}}
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| ** forms focal structure in the cytoplasma membrane {{PubMed|22753055}}
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| ** 13 foci [[FloA]] of are detected in strain 3610 {{PubMed|25909364}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU25380&redirect=T BSU25380]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P54466 P54466]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU25380]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[yqeZ]]-[[floA]]-[[yqfB]]'' {{PubMed|11866510}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yqfA_2617449_2618444_-1 floA] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigW]] {{PubMed|11866510}}
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| * '''Regulation:'''
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| ** expressed under stress conditions ([[SigW]]) {{PubMed|11866510}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 3975 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 3635 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** JS119 (3610 markerless), available in [[Daniel Lopez]]'s lab
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| ** DL1401 (168 ''ΔfloA''::mls), available in [[Daniel Lopez]]'s lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| ** GK192 (3610 ''lacA''::P''floT-yfp''(mls)), available in [[Daniel Lopez]]'s lab
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| ** JS136 (3610 ''amyE::floT''-gfp(spc)), available in [[Daniel Lopez]]'s lab
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| ** JS134 (3610 ''lacA::floA''-mEos2 (mls)), available in [[Daniel Lopez]]'s lab
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| ** JS167 (3610 ''lacA::floA''-PAmCherry (mls)), available in [[Daniel Lopez]]'s lab
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| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]) for FloA {{PubMed|25909364}}, available in [[Daniel Lopez]]'s lab
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| == Reviews ==
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| <pubmed> 25652542</pubmed>
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| == Original publications ==
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| <pubmed>16629676,11866510,18763711, 20713508 22753055 22882210 23651456 22178969 24222488 25909364 </pubmed>
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| [[Category:Protein-coding genes]]
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