|
|
| Line 1: |
Line 1: |
| * '''Description:''' cell-division inhibitor (septum placement), part of the Min system (with [[DivIVA]], [[MinC]], [[MinJ]]), [[Noc]] and the Min system ensure the efficient utilization of the division site at midcell in by ensuring [[FtsZ|Z ring]] placement <br/><br/>
| | {{#bank: MinD}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''minD''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''divIVB1 ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || cell-division inhibitor
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || septum placement
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27990 minD]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MinD MinD]
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 4.984
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 804 bp, 268 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoIVFA]]'', ''[[minC]]''
| |
| |-
| |
| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU27990 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU27990 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU27990 DNA_with_flanks]
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:minD_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=minD_2857776_2858582_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:minD_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27990]]
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[cell division]]}},
| |
| {{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}},
| |
| {{SubtiWiki category|[[membrane proteins]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
| {{SubtiWiki regulon|[[SigH regulon]]}},
| |
| {{SubtiWiki regulon|[[SigM regulon]]}}
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU27990
| |
|
| |
| ===Phenotypes of a mutant ===
| |
| * a ''[[yvcL]] [[minC]]-[[minD]]'' mutant grows poorly, and the cells are filamentous {{PubMed|24097947}}
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU27990&redirect=T BSU27990]
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mreBCD-minCD.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10330]
| |
|
| |
| === Additional information===
| |
| * A mutation was found in this gene after evolution under relaxed selection for sporulation {{PubMed|21821766}}
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:''' The Min system prevents minicell formation adjacent to recently completed division sites by promoting the disassembly of the cytokinetic ring, thereby ensuring that cell division occurs only once per cell cycle {{PubMed|20352045}}
| |
|
| |
| * '''Protein family:''' MinD subfamily (according to Swiss-Prot)
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''[[Domains]]:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''[[Cofactors]]:'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
| ** ([[MinC]]-[[MinD]])-[[MinJ]]-[[DivIVA]] {{PubMed|20352045}}
| |
|
| |
| * '''[[Localization]]:'''
| |
| ** polar/ septal at the cell membrane {{PubMed|20566861}}
| |
| ** membrane binding/ polar localization depends on the proton motive force {{PubMed|20566861}}
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU27990&redirect=T BSU27990]
| |
|
| |
| * '''Structure:'''
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/Q01464 Q01464]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU27990]
| |
|
| |
| * '''E.C. number:'''
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
| * '''Operon:'''
| |
| ** ''[[radC]]-[[mreB]]-[[mreC]]-[[mreD]]-[[minC]]-[[minD]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 PubMed]
| |
| ** ''[[mreB]]-[[mreC]]-[[mreD]]-[[minC]]-[[minD]]'' {{PubMed|8459776}}
| |
| ** ''[[minC]]-[[minD]]'' {{PubMed|8459776}}
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=minD_2857776_2858582_-1 minD] {{PubMed|22383849}}
| |
|
| |
| * '''[[Sigma factor]]:'''
| |
| ** ''[[radC]]'': [[SigM]] {{PubMed|18179421}}
| |
| ** ''[[minC]]'': [[SigH]] {{PubMed|8459776}}
| |
|
| |
| * '''Regulation:'''
| |
| ** expression is reduced in a [[SigV]] mutant {{PubMed|21926231}}
| |
|
| |
| * '''Regulatory mechanism:'''
| |
|
| |
| * '''Additional information:'''
| |
| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 716 {{PubMed|24696501}}
| |
| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 813 {{PubMed|24696501}}
| |
| ** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 3544 {{PubMed|21395229}}
| |
| ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1500 {{PubMed|21395229}}
| |
| ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 6195 {{PubMed|21395229}}
| |
|
| |
| =Biological materials =
| |
| * '''Mutant:'''
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
| ==Reviews==
| |
| <pubmed> 19884039 24367361</pubmed>
| |
| ==Original Publications==
| |
| <pubmed>8459776,19019154,11886553,9808628,1400224,12368265,10411726,15317782,12492861, 20352045, 11886553 20566861 18179421 21821766 22457634 21926231 24097947 25374563 </pubmed>
| |
|
| |
| [[Category:Protein-coding genes]]
| |
