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| * '''Description:''' L-threonylcarbamoyl-AMP synthase, biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A) (together with [[TsaB]]-[[TsaD]]-[[TsaE]]) <br/><br/>
| | {{#bank: TsaC}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''tsaC''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipc-29d, ywlC ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || L-threonylcarbamoyl-AMP synthase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36950 tsaC]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 5.82
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1038 bp, 346 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywlD]]'', ''[[ywlB]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36950 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36950 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36950 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ywlC_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywlC_3792969_3794009_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:ywlC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36950]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[translation]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[DnaA regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU36950
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36950&redirect=T BSU36950]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10939]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** conversion of L-threonine, bicarbonate/CO2 and ATP to give the intermediate L-threonylcarbamoyl-AMP (TC-AMP) and pyrophosphate as products {{PubMed|23072323}}
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| ** biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A), a universal modification found at position 37 of ANN decoding tRNAs, which imparts a unique structure to the anticodon loop enhancing its binding to ribosomes in vitro (shown for the corresponding ''E. coli'' protein YrdC) {{PubMed|19287007}}
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| * '''Protein family:''' SUA5 family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU36950&redirect=T BSU36950]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=1HRU 1HRU] (the homolog from ''E. coli'') {{PubMed|19287007}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P39153 P39153]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU36950]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''''' [[tsaC]]'' {{PubMed|22383849}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywlC_3792969_3794009_-1 tsaC] {{PubMed|22383849}}
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| * '''Sigma factor:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| ** [[DnaA]]: transcription repression {{PubMed|17932079}}
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| * '''Additional information:'''
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 209 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>17005971 17932079 19287007 23072323 22383849 26020636</pubmed>
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| [[Category:Protein-coding genes]]
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