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| * '''Description:''' Fe(II) efflux pump, P1B4 -type ATPase, protects the cell against iron intoxication<br/><br/>
| | {{#bank: PfeT}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''zosA''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ykvW''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || Fe(II) efflux pump
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| |style="background:#ABCDEF;" align="center"|'''Function''' || protection against toxic iron
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13850 pfeT]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=PfeT PfeT]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 68 kDa, 4.934
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1911 bp, 637 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[stoA]]'', ''[[papB]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU13850 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU13850 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU13850 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ykvW_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=zosA_1451371_1453284_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:zosA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13850]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[transporters/ other]]}},
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| {{SubtiWiki category|[[iron metabolism]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[PerR regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU13850
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| ===Phenotypes of a mutant ===
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| ** sensitivity to iron, this can be suppressed by low levels of Mn(II) {{PubMed|26261021}}
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| ** reduced genetic competence, can be rescued by the addition of excess zinc {{PubMed|21813502}}
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| ** reduced expression of ''[[comK]]'', control is exerted at the post-transcriptional level and can be rescued by the addition of excess zinc {{PubMed|21813502}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13850&redirect=T BSU13850]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13325]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + H<sub>2</sub>O + Zn<sup>2+</sup>(In) = ADP + phosphate + Zn<sup>2+</sup>(Out) (according to Swiss-Prot) P-type zinc-transporting ATPase [http://www.ncbi.nlm.nih.gov/sites/entrez/12180919 PubMed]
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| * '''Protein family:''' Type IB subfamily (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:''' cell membrane (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13850&redirect=T BSU13850]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O31688 O31688]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU13850]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/3.6.3.5 3.6.3.5]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''pfeT'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12180919 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=zosA_1451371_1453284_1 pfeT] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12180919 PubMed]
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| * '''Regulation:''' induced by hydrogen peroxide ([[PerR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12180919 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[PerR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12180919 PubMed]
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| * '''Additional information:'''
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| ** A [[ncRNA]] is predicted between ''[[stoA]]'' and ''[[zosA]]'' {{PubMed|20525796}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>14563870,12426338,12486061,12180919,12779235, 21813502,12180919,12180919, 20525796 26261021</pubmed>
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| [[Category:Protein-coding genes]]
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