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| * '''Description:''' diadenylate cyclase, synthesis of c-di-AMP in vegetative cells<br/><br/>
| | {{#bank: CdaA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''cdaA''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ybbP, ybbQ ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || diadenylate cyclase
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of c-di-AMP
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01750 cdaA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=CdaA CdaA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=cdaA cdaA]'''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 30 kDa, 8.074
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 819 bp, 273 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rsiW]]'', ''[[cdaR]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU01750 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU01750 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU01750 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ybbP_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ybbP_196213_197034_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:ybbP_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01750]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[membrane proteins]]}},
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| {{SubtiWiki category|[[metabolism of signalling nucleotides]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU01750
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| ===Phenotypes of a mutant ===
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| * inactivation of ''[[cdaA]]'' results in severe beta-lactam sensitivity {{PubMed|22211522}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01750&redirect=T BSU01750]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11577]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** synthesis of c-di-AMP from two molecules of ATP {{PubMed|23192352}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):''' [[CdaS]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| ** contains a [[DAC domain]] involved in the synthesis of c-di-AMP {{PubMed|21566650}}
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| ** the interaction with [[CdaR]] stimulates the diadenylate cyclase activity of [[CdaA]] {{PubMed|23192352}}
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[CdaA]]-[[CdaR]], to control [[CdaA]] activity {{PubMed|23192352}}
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| ** [[CdaA]]-[[GlmM]] {{PubMed|26240071}}
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| * '''[[Localization]]:'''
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| ** cell membrane (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU01750&redirect=T BSU01750]
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| * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=4RV7 4RV7] (the [[DAC domain]] and C-terminal domain of CdaA from ''Listeria monocytogenes'' (aa 101 - 273), 65% identity) {{PubMed|25605729}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/Q45589 Q45589]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU01750]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[cdaA]]-[[cdaR]]-[[glmM]]-[[glmS]]'' {{PubMed|23192352}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ybbP_196213_197034_1 cdaA] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|22211522}}
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:''' the ''[[cdaA]]-[[cdaR]]'' part of the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 60 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 239 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** GP94 (D''[[cdaA]]''::''spc''), available in [[Jörg Stülke]]'s lab
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| ** GP997 (''[[cdaA]]''::''cat''), available in [[Jörg Stülke]]'s lab
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| ** GP985 (''[[cdaA]]''-''[[cdaR]]''::''cat''), available in [[Jörg Stülke]]'s lab
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| * '''Expression vector:'''
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| ** expression of native ''cdaA'' in ''B. subtilis'': pGP1960 (in [[pBQ200]]), available in [[Jörg Stülke]]'s lab
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| ** expression of ''cdaA''-Strep in ''B. subtilis'' suitable for [[SPINE]]: pGP1986 (in [[pGP382]]), available in [[Jörg Stülke]]'s lab
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| ** IPTG inducible expression of ''cdaA''-Strep in ''E. coli'': pGP2564 (in [[pGP574]]), available in [[Jörg Stülke]]'s lab
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| * '''lacZ fusion:''' GP1339 (cat) based on [[pAC6]], available in [[Jörg Stülke]]'s lab
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab. Respective plasmid: pGP1990.
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| * '''FLAG-tag construct:'''
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| ** GP1381 ''cdaA-3xFLAG ermC'' (based on [[pGP1087]]), available in [[Jörg Stülke]]'s lab
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 18714086 25869574 </pubmed>
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| ==Original publications==
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| <pubmed>12884008,21566650, 23192352 22211522 25605729 25616256 26240071 </pubmed>
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| [[Category:Protein-coding genes]]
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