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| * '''Description:''' cyclic di-AMP phosphodiesterase
| | {{#bank: GdpP}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''gdpP''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yybT ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || c-di-AMP phosphodiesterase
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || control of sporulation initiation, cell wall homeostasis
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40510 gdpP]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=GdpP GdpP]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 74 kDa, 4.865
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1977 bp, 659 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rplI]]'', ''[[yybS]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU40510 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU40510 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU40510 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:yybT_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yybT_4163643_4165622_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:gdpP_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40510]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[metabolism of signalling nucleotides]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU40510
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| ===Phenotypes of a mutant ===
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| * inactivation of ''[[gdpP]]'' strongly restores beta-lactam resistance in a ''[[sigM]]'' mutant {{PubMed|22211522}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40510&redirect=T BSU40510]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yybST-rplI.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10010]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** cyclic dinucleotide phosphodiesterase, hydrolysis of di-cyclic AMP {{PubMed|19901023}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| ** two transmembrane domains at the N-terminus {{PubMed|19901023}}
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| ** a domain that shares minimum sequence homology with Heme-binding [[PAS domain|Per-ARNT-Sim (PAS) domains]] (80 aa) {{PubMed|21257773}}
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| ** degenerate GGDEF domain (aa 173 - 301) {{PubMed|19901023}}
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| ** DHH/ DHHA1 phosphodiesterase domain {{PubMed|19901023}}
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| ** ppGpp inhibits cyclic dinucleotide phosphodiesterase activity {{PubMed|19901023}}
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| ** Heme binding suppresses phosphodiesterase activity {{PubMed|21257773}}
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU40510&redirect=T BSU40510]
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| * '''Structure:'''
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| ** [http://www.rcsb.org/pdb/explore/explore.do?structureId=2M1C 2M1C] (N-terminal PAS domain of GdpP from ''Geobacillus thermodenitrificans'') {{PubMed|23504327}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P37484 P37484]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU40510]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[yybS]]-[[gdpP]]-[[rplI]]'' (according to [http://dbtbs.hgc.jp/COG/prom/yybST-rplI.html DBTBS])
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yybT_4163643_4165622_-1 gdpP] {{PubMed|22383849}}
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| * '''Sigma factor:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** there is an internal [[SigD]]-dependent antisense transcript (as-''[[gdpP]]'') {{PubMed|22956758}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** GP998 (''[[gdpP]]''::''spc''), available in [[Jörg Stülke]]'s lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 25869574</pubmed>
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| ==Original publications==
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| <pubmed> 19901023 21257773 21566650 23504327 22211522,22956758 25616256</pubmed>
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| ==GdpP homologs in other organisms==
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| <pubmed> 18502872 </pubmed>
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| [[Category:Protein-coding genes]]
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