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| * '''Description:''' pyruvate carboxylase <br/><br/>
| | {{#bank: PycA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''pycA''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylaP ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || pyruvate carboxylase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || replenishment of the oxaloacetate pool
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14860 pycA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=pycA pycA]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 127 kDa, 5.407
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 3444 bp, 1148 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ftsW]]'', ''[[ctaA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU14860 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU14860 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU14860 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:pycA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pycA_1554185_1557631_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:pycA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14860]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[carbon core metabolism]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[stringent response]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU14860
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU14860&redirect=T BSU14860]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ftsW-pycA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12660]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:''' biotin
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| ** membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
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| ** cytoplasm (homogeneously distributed throughout the cell) {{PubMed|24825009}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU14860&redirect=T BSU14860]
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3BG5 3BG5] (''S. aureus'')
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/Q9KWU4 Q9KWU4]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU14860]
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| * '''E.C. number:''' 6.4.1.1
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| === Additional information===
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| PycA binds to StrepTactin, and may be co-purified when purifying Strep-tagged proteins by [[SPINE]].
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| PycA is subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
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| =Expression and regulation=
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| * '''Operon:''' ''[[pycA]]'' {{PubMed|20081037}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pycA_1554185_1557631_1 pycA] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|20081037}}
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| * '''Regulation:'''
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| ** subject to positive stringent control upon lysine starvation {{PubMed|20081037}}
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| * '''Regulatory mechanism:'''
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| ** stringent response: due to presence of adenines at +1 and +2 positions of the transcript {{PubMed|20081037}}
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| * '''Additional information:''' subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 2222 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 6831 {{PubMed|24696501}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1602 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 907 {{PubMed|21395229}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 2037 {{PubMed|21395229}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** GP793 (cat), available in [[Jörg Stülke]]'s lab
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| * '''Expression vector:'''
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| ** pGP1289 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>18613815 12769720 10229653 9597748 7780827 </pubmed>
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| ==Original publications==
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| <pubmed>18763711, 20081037 24825009 25755103</pubmed>
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| [[Category:Protein-coding genes]]
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