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| * '''Description:''' spore coat protein, phospholipase implicated in spore germination <br/><br/>
| | {{#bank: LipC}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''lipC''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ycsK''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || spore coat phospholipase B
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| |style="background:#ABCDEF;" align="center"|'''Function''' || spore [[germination]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU04110 lipC]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=lipC lipC]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23 kDa, 5.839
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 639 bp, 213 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[kipR]]'', ''[[yczI]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU04110 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU04110 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU04110 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ycsK_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=lipC_462431_463072_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:lipC_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU04110]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[utilization of lipids]]}},
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| {{SubtiWiki category|[[sporulation proteins]]}},
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| {{SubtiWiki category|[[germination]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[KipR regulon]]}},
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| {{SubtiWiki regulon|[[SigK regulon]]}},
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| {{SubtiWiki regulon|[[TnrA regulon]]}},
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| {{SubtiWiki regulon|[[GerE regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU04110
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| ===Phenotypes of a mutant ===
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| * defective in L-alanine-stimulated germination {{PubMed|20057119}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU04110&redirect=T BSU04110]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ycsFGI-kipIAR-ycsK.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11232]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' cleaves the fatty acids at the sn-1 and sn-2 positions of phospholipids {{PubMed|20057119}}
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| * '''Protein family:''' 'GDSL' lipolytic enzyme family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| ** spore coat (basement), localization depends on [[SpoIVA]] {{PubMed|22171814,17220230,20057119}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU04110&redirect=T BSU04110]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P42969 P42969]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU04110]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| ** ''[[ycsF]]-[[ycsG]]-[[ycsI]]-[[kipI]]-[[kipA]]-[[kipR]]-[[lipC]]'' {{PubMed|9334321}}
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| ** ''lipC'' (according to [http://dbtbs.hgc.jp/COG/prom/ycsFGI-kipIAR-ycsK.html DBTBS])
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=lipC_462431_463072_1 lipC] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| ** ''lipC'': [[SigK]] {{PubMed|15699190,17220230}}
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| * '''Regulation:'''
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| ** ''lipC'': expressed four hours after onset of sporulation ([[GerE]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/17220230 PubMed]
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| * '''Regulatory mechanism:'''
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| ** ''[[ycsF]]'': [[KipR]]: transcription repression {{PubMed|9334321}}
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| ** ''[[ycsF]]'': [[TnrA]]: transcription activation {{PubMed|9334321}}
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| ** [[GerE]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/17220230 PubMed]
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| == Reviews ==
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| <pubmed>23202530</pubmed>
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| == Original publications ==
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| <pubmed> 17220230 9334321 20057119 22171814</pubmed>
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| [[Category:Protein-coding genes]]
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