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| * '''Description:''' uricase, urate oxidase <br/><br/>
| | {{#bank: PucL}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''pucL''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yunL ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || uricase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || purine utilization
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU32450 pucL]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=pucL pucL]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 56 kDa, 5.484
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1482 bp, 494 aa
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pucK]]'', ''[[pucM]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU32450 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU32450 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU32450 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:pucL_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pucL_3333162_3334646_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:pucL_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU32450]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[utilization of nucleotides]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[PucR regulon]]}},
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| {{SubtiWiki regulon|[[TnrA regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU32450
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU32450&redirect=T BSU32450]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pucRJKLM.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13986]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate = (S)-allantoin + CO<sub>2</sub> (according to Swiss-Prot)
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU32450&redirect=T BSU32450]
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1J2G 1J2G] (with 8-Azaxanthine from ''Bacillus sp.'', 66% identity, 79% similarity)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O32141 O32141]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU32450]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/1.7.3.3 1.7.3.3]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| ** ''[[pucR]]-[[pucJ]]-[[pucK]]-[[pucL]]-[[pucM]]'' {{PubMed|12823818}}
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| ** ''[[pucJ]]-[[pucK]]-[[pucL]]-[[pucM]]'' {{PubMed|12029039}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pucL_3333162_3334646_1 pucL] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| ** ''[[pucJ]]'': [[SigA]] {{PubMed|12029039}}
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| * '''Regulation:'''
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| ** expressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) {{PubMed|12823818}}
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| ** induced in the presence of purine nucleotides (inducer: allantoin) ([[PucR]]) {{PubMed|12029039}}
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| * '''Regulatory mechanism:'''
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| ** [[TnrA]]: transcription activation {{PubMed|12823818}}
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| ** [[PucR]]: transcription activation {{PubMed|12029039}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>11344136,12823818,12029039,12823818,12029039, 20168977 </pubmed>
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| [[Category:Protein-coding genes]]
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