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| * '''Description:''' gamma-amino butyric acid permease and minor proline permease<br/><br/>
| | {{#bank: GabP}} |
| {| align="right" border="1" cellpadding="2" | |
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''gabP''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''nrg-21 ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || gamma-amino butyric acid permease
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || utilization of gamma-amino butyric acid
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06310 gabP]
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=gabP gabP]'''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 9.45
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1407 bp, 469 aa
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cotA]]'', ''[[ydzX]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU06310 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU06310 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU06310 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:gabP_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gabP_685155_686564_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:gabP_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06310]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[transporters/ other]]}},
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| {{SubtiWiki category|[[utilization of amino acids]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CodY regulon]]}},
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| {{SubtiWiki regulon|[[TnrA regulon]]}}
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU06310
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| ===Phenotypes of a mutant ===
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| * defective in the utilization of gamma-amino butyrate {{PubMed|24142252}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU06310&redirect=T BSU06310]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/gabP.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11328]
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| === Additional information===
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|
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** uptake of gamma-amino butyrate {{PubMed|24142252}}
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| ** minor transporter for proline (allows growth with proline in the absence of the major [[transporters]] [[PutP]] and [[OpuE]]) {{PubMed|24142252}}
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| * '''Protein family:'''
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| ** secondary amino acid transporter
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| ** [[APC superfamily of transport proteins|amino acid, polyamine, organocation (APC) superfamily of transporters]], [http://www.tcdb.org/search/result.php?tc=2.A.3 TC 2.A.3]
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| * '''Paralogous protein(s):''' [[AapA]], [[HutM]], [[RocC]], [[RocE]], [[YbgF]], [[YbxG]], [[YdgF]], [[YtnA]], [[YvbW]]
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|
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| ** K(M) for gamma-amino butyrate: 77 +/- 17 µM {{PubMed|24142252}}
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| ** K(M) for proline: 700 +/- 330 µM {{PubMed|24142252}}
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| ** cell membrane (according to Swiss-Prot)
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU06310&redirect=T BSU06310]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P46349 P46349]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU06310]
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| * '''[http://www.tcdb.org/ T.C.] number:''' [http://www.tcdb.org/search/result.php?tc=2.A.3.1.5 2.A.3.1.5]
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|
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''gabP'' {{PubMed|8951816}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gabP_685155_686564_-1 gabP] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|8951816}}
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| * '''Regulation:'''
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| ** expressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8951816 PubMed]
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| ** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8951816 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[TnrA]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/8951816 PubMed]
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| ** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/8951816 PubMed]
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 178 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** available in [[Erhard Bremer]]'s lab {{PubMed|24142252}}
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>8951816, 9677314, 24142252 </pubmed>
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| [[Category:Protein-coding genes]]
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