KinB

• Description: two-component sensor kinase, phosphorylates Spo0F, part of the phosphorelay, senses changes in respiratory activity
  
  

Gene name	kinB
Synonyms
Essential	no
Product	two-component sensor kinase
Function	initiation of sporulation
Gene expression levels in SubtiExpress: kinB
Interactions involving this protein in SubtInteract: KinB
Function and regulation of this protein in SubtiPathways: kinB
MW, pI	47 kDa, 6.682
Gene length, protein length	1287 bp, 429 aa
Immediate neighbours	patB, kapB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, transcription factors and their control, phosphorelay, membrane proteins, phosphoproteins

This gene is a member of the following regulons

CodY regulon, stringent response

The gene

Basic information

• Locus tag: BSU31450

Database entries

• BsubCyc: BSU31450

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • autophosphorylation, phosphorylation of Spo0F
  • mainly active in the older, inner regions of a colony (with KinA) PubMed
  • sensing potassium level in the medium during initiation of sliding sliding PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • six transmembrane segments, C-terminal histidine phosphotransferase domain
  • selectivity filter sequence of potassium channels that is required for sliding but not for sporulation PubMed

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:
  • activity is triggered at low respiratory activity, this depends on a functional interaction with the respiration apparatus PubMed

• Interactions:
  • KinB-Sda
  • KinB-Spo0F
  • KinB-CtaC PubMed
  • KinB-QcrC PubMed

• Localization: cell membrane (integral membrane protein) PubMed

Database entries

• BsubCyc: BSU31450

• Structure: 3D36 (from G. stearothermophilus, complex with Sda)

• UniProt: Q08430

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: kinB-kapB PubMed

• Expression browser: kinB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • induced upon addition of decoyinine (positive stringent response) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • JH19980 (kinB::tet) PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

<pubmed>26152584,8576055,16166384,9299348,8497199,10094672,9426145,11069677,12618455,11902725,12618455,7592498, 21097618 19101565 23378509 23599347 26152584</pubmed>