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| * '''Description:''' [[protein kinases and phosphatases|protein arginine kinase]], adaptor protein, modulator of [[CtsR]]-dependent repression <br/><br/>
| | {{#bank: McsB}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''mcsB''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yacI ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[protein kinases and phosphatases|protein arginine kinase]]
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || control of [[CtsR]] activity
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00850 mcsB]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=McsB McsB]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mcsB mcsB]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 5.068
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1089 bp, 363 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mcsA]]'', ''[[clpC]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00850 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00850 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00850 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:mcsB_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mcsB_102484_103575_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:mcsB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00850]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[protein modification]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[sporulation proteins]]}},
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| {{SubtiWiki category|[[general stress proteins (controlled by SigB)]]}},
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| {{SubtiWiki category|[[heat shock proteins]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CtsR regulon]]}},
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| {{SubtiWiki regulon|[[SigB regulon]]}},
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| {{SubtiWiki regulon|[[SigF regulon]]}}
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU00850
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00850&redirect=T BSU00850]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ctsR-mcsAB-clpC-radA-yacK.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10147]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' targets non-functional CtsR for degradation by [[ClpP]]/[[ClpC]] {{PubMed|20852588}}
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| * '''Protein family:''' ATP:guanido phosphotransferase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| * '''Modification:'''
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| ** autophosphorylation on specific arginine residues {{PubMed|20852588,21622759}}, dephosphorylation by [[YwlE]] {{PubMed|20852588,21622759}}
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| ** autophosphorylation stimulates McsB activity as adaptor protein for [[ClpC]] {{PubMed|21622759}}
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[McsA]]-[[McsB]]-[[CtsR]], [[McsB]]-[[CtsR]] {{PubMed|20852588}}, [[McsA]]-[[McsB]]
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| ** [[McsB]]-[[YwlE]] {{PubMed|20852588}}
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| ** [[McsB]]-[[ClpP]]/[[ClpC]] (rapid degradation of phosphorylated McsB) {{PubMed|20852588}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00850&redirect=T BSU00850]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P37570 P37570]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU00850]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[ctsR]]-[[mcsA]]-[[mcsB]]-[[clpC]]-[[radA]]-[[disA]]'' [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mcsB_102484_103575_1 mcsB] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed], [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed1] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed2], [[SigF]] {{PubMed|16497325}}
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| * '''Regulation:'''
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| ** expressed early during sporulation in the forespore ([[SigF]]) {{PubMed|16497325}}
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| ** induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed]
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| ** induced by heat ([[CtsR]]) [http://www.ncbi.nlm.nih.gov/pubmed/9987115 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[CtsR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9987115 PubMed]
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 142 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** ''mcsB::aphA3'' availbale from the Gerth lab
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| ** mcsBC167S::spec available from the Gerth lab
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| ** GP1457 (''mcsB''::''aphA3''), available in [[Jörg Stülke]]'s lab
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| ** BP69 (spc), available in [[Fabian Commichau]]'s lab
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| * '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
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| ** expression of native ''mcsB'' in ''B. subtilis'': pBP529186 (in [[pBQ200]]), available in [[Fabian Commichau]]'s lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:''' available in Gerth lab
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| =Labs working on this gene/protein=
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| * [[Ulf Gerth]], Greifswald, Germany
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| * [[Fabian Commichau]] Göttingen, Germany
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 23375660 19609260</pubmed>
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| ==Original Publications==
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| <pubmed>17380125,16163393,19498169 11179229,9987115, 8793870, 16497325,19226326, 9987115, 11544224, 20852588 21622759 22517742 24825175 24263382 25610436</pubmed>
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| [[Category:Protein-coding genes]]
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