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| * '''Description:''' modulation of [[CheA]] activity in response to attractants <br/><br/>
| | {{#bank: CheV}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''cheV''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[CheA]] modulator
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| |style="background:#ABCDEF;" align="center"|'''Function''' || control of [[CheA]] activity
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14010 cheV]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=CheV CheV]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 4.617
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 909 bp, 303 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykzT]]'', ''[[kre]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU14010 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU14010 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU14010 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:cheV_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cheV_1473605_1474516_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:cheV_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14010]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[transcription factors and their control]]}}, {{SubtiWiki category|[[phosphoproteins]]}},
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| {{SubtiWiki category|[[motility and chemotaxis]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[SigD regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU14010
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| ===Phenotypes of a mutant ===
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| * ''[[cheV]] [[cheW]]'' double mutants exhibit complete loss of chemotaxis {{PubMed|21098025}}
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| * not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation {{PubMed|26122431}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU14010&redirect=T BSU14010]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cheV.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10823]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:'''
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| * '''Paralogous protein(s):''' [[CheW]] (N-terminal domain of CheV)
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:''' N-terminal [[CheW]]-like domain, C-terminal two-component receiver domain {{PubMed|11553614}}
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| * '''Modification:''' the C-terminal two-component receiver domain is phosphorylated on a Asp residue by [[CheA]] {{PubMed|11553614}}
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[CheA]]-[[CheY]] {{PubMed|11553614}}
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| * '''[[Localization]]:'''
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| ** forms lateral clusters (phosphorylated form), but in the presence of high asparagine concentration (non-phosphorylated form) there is a reversible re-localization to the poles of the cell {{PubMed|21098025}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU14010&redirect=T BSU14010]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P37599 P37599]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU14010]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[cheV]]'' {{PubMed|8169223}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cheV_1473605_1474516_1 cheV] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigD]] {{PubMed|8169223}}
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** in minimal medium, CheV is present with 7,500 +/- 2,000 molecules per cell {{PubMed|21515776}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 1117 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 1764 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** DS70 (''cheV''::''mls'' in NCIB3610) {{PubMed|12864845}}
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| ** TB183 ''amyE''::Phy-''sfgfp'' (''cheV''::''mls'' in NCIB3610 with constitutive expressed ''sfgfp'') {{PubMed|26122431}}
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| ** TB199 ''amyE''::Phy-''mKATE2'' (''cheV''::''mls'' in NCIB3610 with constitutive expressed ''mKATE2'') {{PubMed|26122431}}
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>18774298,</pubmed>
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| ==Original publications==
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| <pubmed> 12864845,26122431, 8169224,8169223,11553614,14731274,21098025, 21515776</pubmed>
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| [[Category:Protein-coding genes]]
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