FliF

• Description: flagellar basal-body M-ring protein, membrane anchor of the basal body
  
  

Gene name	fliF
Synonyms
Essential	no
Product	flagellar basal-body M-ring protein
Function	movement and chemotaxis
Gene expression levels in SubtiExpress: fliF
Interactions involving this protein in SubtInteract: FliF
MW, pI	59 kDa, 4.37
Gene length, protein length	1608 bp, 536 aa
Immediate neighbours	fliE, fliG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16210

Phenotypes of a mutant

• mucoid phenotype due to the overproduction of poly-gamma-glutamate PubMed
• no secretion of FlgM, permanent inhibition of SigD PubMed
• not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed

Database entries

• BsubCyc: BSU16210

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • required for correct localization of FliM PubMed

• Protein family: fliF family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • FliF-FliG PubMed

• Localization:
  • cell membrane PubMed

Database entries

• BsubCyc: BSU16210

• Structure:

• UniProt: P23447

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: part of the fla-che operon (see there for regulation)
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: fliF PubMed

• Sigma factor: SigA PubMed, SigD PubMed

• Regulation: see fla-che operon

• Regulatory mechanism: see fla-che operon

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 68 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 595 PubMed

Biological materials

• Mutant:
  • DS7080 (marker-less in NCIB3610) PubMed
  • TB192 amyE::Phy-sfgfp (marker-less in NCIB3610 with constitutive expressed sfgfp) PubMed
  • TB208 amyE::Phy-mKATE2 (marker-less in NCIB3610 with constitutive expressed mKATE2) PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

<pubmed> 26122431, 1905667,17850253,14651647,18763711, 9657996,8157612,15175317 23190039 24296669 25313396 24386445 25843804</pubmed>