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| * '''Description:''' ATP-dependent Clp protease ATP-binding subunit (class III heat-shock protein) <br/><br/>
| | {{#bank: ClpX}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''clpX''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || ATP-dependent Clp protease ATP-binding subunit
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || protein degradation
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28220 clpX]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=ClpX ClpX]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=clpX clpX]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46 kDa, 4.645
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1260 bp, 420 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[lonB]]'', ''[[tig]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU28220 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU28220 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU28220 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:clpX_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=clpX_2884781_2886043_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:clpX_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28220]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[proteolysis]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CtsR regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU28220
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| ===Phenotypes of a mutant ===
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| * increased thermotolerance due to increased stabiliy of [[Spx]] and thus increased expression of ''[[trxA]]'' {{PubMed|24417481}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28220&redirect=T BSU28220]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/clpX.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11387]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATPase/chaperone
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| * '''Protein family:''' clpX chaperone family (according to Swiss-Prot) ClpX (IP004487) [http://www.ebi.ac.uk/interpro/IEntry?ac=IPR004487 InterPro], AAA+ -type ATPase (IPR013093) [http://www.ebi.ac.uk/interpro/IEntry?ac=IPR013093 InterPro] (PF07724) [http://pfam.sanger.ac.uk/family?acc=PF07724 PFAM]
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| * '''Paralogous protein(s):''' [[ClpC]], [[ClpE]]
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| === Targets of [[ClpX]]-[[ClpP]]-dependent protein degradation ===
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| * [[MgsR]] {{PubMed|22812682}}
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''[[Domains]]:'''
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| ** AAA-ATPase [http://pfam.sanger.ac.uk/family?acc=PF07724 PFAM]
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| ** Zinc finger [http://pfam.sanger.ac.uk/family?acc=PF06689 PFAM]
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| * '''Modification:'''
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| * '''[[Cofactors]]:'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[ClpP]]-[[ClpX]]
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| ** [[Spx]]-[[ClpP]]/[[ClpX]] (degradation of [[Spx]]) {{PubMed|17827297}}
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| * '''[[Localization]]:''' cytoplasmic polar clusters, excluded from the nucleoid, induced clustering upon heat shock, colocalization with [[ClpP]] {{PubMed|18786145,18689473}}
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| [[File:ClpX.jpg ]]
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28220&redirect=T BSU28220]
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| * '''Structure:''' homologue structure resolved [http://www.rcsb.org/pdb/explore/explore.do?structureId=1UM8 1UM8], structural model of ''B. subtilis'' ClpX available from [http://subtiwiki.uni-goettingen.de/wiki/index.php/User:Hstrahl hstrahl]
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P50866 P50866]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU28220]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''clpX'' {{PubMed|11325926}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=clpX_2884781_2886043_-1 clpX] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|8973311}}
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| * '''Regulation:'''
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| ** induced by heat stress ([[CtsR]]) {{PubMed|9852015}}
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| * '''Regulatory mechanism:'''
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| ** [[CtsR]]: transcription repression {{PubMed|9852015}}
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| * '''Additional information:'''
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| ** The mRNA has a long 5' leader region. This may indicate RNA-based regulation {{PubMed|20525796}}
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 1690 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 6358 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:''' ''clpX::kan'', ''clpX::spec'' and ''clpX::cat'' available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen]] Lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:''' C-terminal GFP fusions (both single copy and 2th copy in ''amyE'' locus, also as CFP and YFP variants) available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen]] Lab
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Leendert Hamoen]], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen homepage]
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>23375660 19680248 17302811 23479438,19609260</pubmed>
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| ==Original Publications==
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| <pubmed>12761164 10809708,9643546,11807061,14679237,18689476,16899079,8973311, 19136590 , 11325926 8973311 9852015 18689473 20525796 15948963 18786145 24417481 24942655 25433860 </pubmed>
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| [[Category:Protein-coding genes]]
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