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| * '''Description:''' developmental checkpoint protein, inhibits the autokinase activity of [[KinA]] and [[KinB]], control of the [[phosphorelay]]
| | {{#bank: Sda}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''sda''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || developmental checkpoint protein
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| |style="background:#ABCDEF;" align="center"|'''Function''' || mediates a developmental checkpoint coupling<br/>initiation of sporulation (phosphorylation of [[Spo0A]])<br/>to the function of replication initiation proteins
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25690 sda]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=Sda Sda]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=sda sda]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 6 kDa, 5.605
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 156 bp, 52 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yloV]]'', ''[[yqeF]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25690 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25690 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25690 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:sda_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sda_2647456_2647614_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:sda_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25690]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[phosphorelay]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[DnaA regulon]]}},
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| {{SubtiWiki regulon|[[LexA regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU25690
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| ===Phenotypes of a mutant ===
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| *sporulates at a higher frequency
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| *will sporulate in the presence of replication stress
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU25690&redirect=T BSU25690]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sda.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14183]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Sda is a checkpoint protein that is upregulated in response to replication stress {{PubMed|11207367}}. Sda inhibits the autokinase activity of [[KinA]] (and likely [[KinB]] as well). This in its turn results in reduced levels of [[Spo0A|Spo0A~P]]. Thus, replication stressed cells will not initiate sporulation.
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[KinB]]-[[Sda]] {{PubMed|19465772}}
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| ** [[KinA]]-[[Sda]] {{PubMed|15023339}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU25690&redirect=T BSU25690]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3D36 3D36] (from Geobacillus stearothermophilus, complex with [[KinB]]) {{PubMed|19101565}}, [http://www.rcsb.org/pdb/explore.do?structureId=1PV0 1PV0]
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/Q7WY62 Q7WY62]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU25690]
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| * '''E.C. number:'''
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| === Additional information===
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| * [[Sda]] is rapidly degraded by the [[ClpP|ClpP/X]] system because of some uncharged residues at its C-terminus {{PubMed|16796683}}
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| =Expression and regulation=
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| * '''Operon:''' ''[[sda]]'' {{PubMed|11207367}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sda_2647456_2647614_1 sda] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|11207367}}
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| * '''Regulation:'''
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| ** induced by DNA damage ([[LexA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/16267290 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[DnaA]]: transcription activation under replication stress {{PubMed|17932079}}
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| ** [[LexA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/16267290 PubMed]
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| * '''Additional information:'''
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| ** Sda is rapidly turned over by the [[ClpP|ClpP/X]] system because of some uncharged residues at its C-terminus {{PubMed|16796683}}.
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| ** An [[ncRNA|antisense RNA]] is predicted for ''[[sda]]'' {{PubMed|20525796}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1A1034 (no resistance), {{PubMed|16796683}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1034&Search=1A1034 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Bill Burkholder]] [http://burkholder.stanford.edu/ Homepage]
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 22933559</pubmed>
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| == Original publications ==
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| <pubmed>11207367, 16267290, 19465772, 15023339 16796683 19684115 17932079 17350039 19101565 20525796 26020636</pubmed>
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| [[Category:Protein-coding genes]]
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