OppF

• Description: oligopeptide ABC transporter (ATP-binding protein)
  
  

Gene name	oppF
Synonyms	spo0KE
Essential	no
Product	oligopeptide ABC transporter (ATP-binding protein)
Function	initiation of sporulation, competence development
Gene expression levels in SubtiExpress: oppF
Interactions involving this protein in SubtInteract: OppF
Metabolic function and regulation of this protein in SubtiPathways: oppF
MW, pI	34 kDa, 6.95
Gene length, protein length	915 bp, 305 aa
Immediate neighbours	oppD, yjbB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, utilization of nitrogen sources other than amino acids, genetic competence, phosphorelay, membrane proteins

This gene is a member of the following regulons

CodY regulon, ScoC regulon, TnrA regulon

The gene

Basic information

• Locus tag: BSU11470

Database entries

• BsubCyc: BSU11470

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • required for the uptake of quorum sensing peptides such as PhrH PubMed

• Protein family: ABC transporter domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • (OppD-OppF)-(OppB-OppC)-OppA PubMed

• Localization: attached to the cell membrane (via OppB-OppC) PubMed

Database entries

• BsubCyc: BSU11470

• Structure:

• UniProt: P24137

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: oppA-oppB-oppC-oppD-oppF PubMed

• Expression browser: oppF PubMed

• Sigma factor:

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • repressed during exponential growth (ScoC) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed
  • ScoC: transcription repression PubMed
  • CodY: transcription repression, no derepression occurs, however, in a codY mutant, due to increased repression by ScoC PubMed

• Additional information:
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 365 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

<pubmed>12823818,1901616,10092453,9252573,1899858 21908671,12823818,10383984, 25966844</pubmed>