PrkC

• Description: protein kinase C, induce germination of spores in response to DAP-type, and not to Lys-type cell wall muropeptides
  
  

Gene name	prkC
Synonyms	yloP
Essential	no
Product	protein kinase
Function	germination in response to muropeptides
Gene expression levels in SubtiExpress: prkC
Interactions involving this protein in SubtInteract: PrkC
Metabolic function and regulation of this protein in SubtiPathways: prkC
MW, pI	71 kDa, 4.833
Gene length, protein length	1944 bp, 648 aa
Immediate neighbours	prpC, cpgA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, germination, membrane proteins, phosphoproteins

The gene

Basic information

• Locus tag: BSU15770

Phenotypes of a mutant

• unable to germinate in response to muropeptides PubMed
• reduced growth at high salt PubMed

Database entries

• BsubCyc: BSU15770

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)

• Protein family: protein kinase domain (according to Swiss-Prot)

• Paralogous protein(s):

Proteins phosphorylated by PrkC

CpgA, EF-Tu, YezB PubMed, EF-G PubMed, YwjH, GlnA, Icd, AlsD, HPr PubMed, YkwC PubMed, YvcK PubMed, GpsB PubMed

Extended information on the protein

• Kinetic information:

• Domains:
  • contains three C-terminal PASTA domains (aa 356-424, 425-492, 493-559) (binds muropeptides) PubMed

• Modification: phosphorylation on Thr-290 PubMed, autophosphorylation on multiple threonine residues PubMed

• Cofactors:

• Effectors of protein activity:
  • activated by muropeptides PubMed
  • GpsB, DivIVA, and EzrA are required for stimulation of PrkC activity PubMed

• Interactions:
  • AbrB-PrkC PubMed
  • YvcK-PrkC PubMed
  • GpsB-PrkC PubMed

• Localization:
  • inner spore membrane PubMed
  • membrane PubMed
  • division septum PubMed

Database entries

• BsubCyc: BSU15770

• Structure:
  • 3PY3 (entire extra-cellular region of PrkC from Staphylococcus aureus) PubMed
  • 4X3F (intracellular domain of the Mycobacterium tuberculosis enzyme, 36% identity, 68% similarity) PubMed

• UniProt: O34507

• KEGG entry: [2]

• E.C. number: 2.7.11.1

Expression and regulation

• Operon: prpC-prkC-cpgA PubMed

• Expression browser: prkC PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP576 (spc), OMG302 (aphA3), available in Stülke lab
  • 1A820 ( prkC::erm), PubMed, available at BGSC
  • 1A963 (no resistance), PubMed, available at BGSC
  • 1A964 (no resistance), PubMed, available at BGSC

• Expression vector:
  • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP832, available in Stülke lab
  • for expression/ purification of the kinase domain from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP849, available in Stülke lab
  • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1001, available in Stülke lab
  • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP825, available in Stülke lab

• lacZ fusion: pGP829 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

<pubmed> 20972452 21372323 </pubmed>

Phosphorylation of PrkC

<pubmed>12842463 20563625</pubmed>

Targets of PrkC-dependent phosphorylation

<pubmed>19246764, 20070526 ,20389117 24390483 24731262 25012659 25278935 25845974</pubmed>

Phsiological role of PrkC

<pubmed>12399479, 12406230, 19246764, 12842463 , 18984160 </pubmed>

Expression of PrkC

<pubmed>16025310 24752279</pubmed>

Structure/ biochemistry of PrkC

<pubmed> 21208192 22111897 23793375 25668224 25586004</pubmed>