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| Line 1: |
Line 1: |
| * '''Description:''' required for removal of [[ponA|PBP1]] from the cell pole after completion of cell pole maturation <br/><br/>
| | {{#bank: GpsB}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''gpsB''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ypsB ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || cell division protein, member of the divisome
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || cell division, cell elongation
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22180 gpsB]
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=GpsB GpsB]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 5.629
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 294 bp, 98 aa
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rnpB]]'', ''[[ypsA]]''
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| |-
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22180 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22180 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22180 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ypsB_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gpsB_2331779_2332075_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:gpsB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22180]]
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| |-
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| |}
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|
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[cell division]]}},
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| {{SubtiWiki category|[[membrane proteins]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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|
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|
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| =The gene=
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|
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| === Basic information ===
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| * '''Locus tag:''' BSU22180
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|
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| ===Phenotypes of a mutant ===
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| * synthetic lethal phenotype with a ''[[ftsA]]'' mutation {{PubMed|18776011}}; synthetic sick phenotype when combined with an ''[[ezrA]]'' mutation {{PubMed|18363795}}
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|
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU22180&redirect=T BSU22180]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11454]
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|
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:'''
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| * '''Paralogous protein(s):''' [[DivIVA]]
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|
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:''' contains a [[DivIVA]]-like N-terminal domain
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| * '''Modification:''' phosphorylation on (Ser-73 OR Thr-75) [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[GpsB]]-[[EzrA]] {{PubMed|18363795}}
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| ** [[GpsB]]-[[PonA]] {{PubMed|18363795}}
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| ** [[GpsB]]-[[MreC]] {{PubMed|18363795}}
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|
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| * '''[[Localization]]:'''
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| ** dynamic, shuttles between lateral membrane and the divison septum [http://www.ncbi.nlm.nih.gov/sites/entrez/18363795 PubMed], localizes to the divisome after Z-ring formation {{PubMed|18776011}}
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|
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU22180&redirect=T BSU22180]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P50839 P50839]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22180]
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| * '''E.C. number:'''
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| === Additional information===
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|
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| =Expression and regulation=
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| * '''Operon:'''
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=gpsB_2331779_2332075_-1 gpsB] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 1059 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 1205 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1A934 ( ''gpsB''::''kan''), {{PubMed|18776011}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A934&Search=1A934 BGSC]
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| ** 1A970 ( ''gpsB''::''kan''), {{PubMed|18776011}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A970&Search=1A970 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>18776011,, 19429628, 17218307, 18363795 </pubmed>
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| [[Category:Protein-coding genes]]
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