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* '''Description:''' spore coat polysaccharide synthesis <br/><br/>
{{#bank: SpsF}}


{| align="right" border="1" cellpadding="2"
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|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''spsF''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-68d ''
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
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|style="background:#ABCDEF;" align="center"|'''Function''' || spore coat polysaccharide synthesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37860 spsF]
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.515 
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 717 bp, 239 aa
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spsG]]'', ''[[spsE]]''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37860 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37860 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37860 DNA_with_flanks]
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:spsF_context.gif]]
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spsF_3887026_3887748_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:spsF_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37860]]
|-
|}
__TOC__
<br/><br/><br/><br/>
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<br/><br/>
= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[sporulation proteins]]}}
= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[GerE regulon]]}},
{{SubtiWiki regulon|[[SigK regulon]]}}
=The gene=
=== Basic information ===
* '''Locus tag:''' BSU37860
===Phenotypes of a mutant ===
=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU37860&redirect=T BSU37860]
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sps.html]
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10614]
=== Additional information===
=The protein=
=== Basic information/ Evolution ===
* '''Catalyzed reaction/ biological activity:'''
* '''Protein family:''' CMP-NeuNAc synthetase family (according to Swiss-Prot)
* '''Paralogous protein(s):'''
=== Extended information on the protein ===
* '''Kinetic information:'''
* '''Domains:'''
* '''Modification:'''
* '''Cofactor(s):'''
* '''Effectors of protein activity:'''
* '''[[SubtInteract|Interactions]]:'''
* '''[[Localization]]:'''
=== Database entries ===
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU37860&redirect=T BSU37860]
* '''Structure:'''
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39626 P39626]
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU37860]
* '''E.C. number:'''
=== Additional information===
=Expression and regulation=
* '''Operon:''' ''[[spsA]]-[[spsB]]-[[spsC]]-[[spsD]]-[[spsF]]-[[spsG]]-[[spsI]]-[[spsJ]]-[[spsK]]-[[spsL]]'' {{PubMed|9353933}}
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spsF_3887026_3887748_-1 spsF] {{PubMed|22383849}}
* '''[[Sigma factor]]:''' [[SigK]] {{PubMed|15383836}}
* '''Regulation:'''
** expressed late during sporulation in the mother cell ([[SigK]], [[GerE]]) {{PubMed|25239894,15383836}}
* '''Regulatory mechanism:'''
** [[GerE]]: transcription activation {{PubMed|25239894,15383836}}
* '''Additional information:'''
=Biological materials =
* '''Mutant:'''
* '''Expression vector:'''
       
* '''lacZ fusion:'''
* '''GFP fusion:'''
* '''two-hybrid system:'''
* '''Antibody:'''
=Labs working on this gene/protein=
=Your additional remarks=
=References=
<pubmed>9353933 15383836 25239894</pubmed>
[[Category:Protein-coding genes]]

Latest revision as of 14:11, 6 April 2015