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| * '''Description:''' frataxin-like protein, required for the biosynthesis of iron-sulfur clusters <br/><br/>
| | {{#bank: Fra}} |
| {| align="right" border="1" cellpadding="2" | |
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''fra''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ydhG ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || frataxin-like protein
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| |style="background:#ABCDEF;" align="center"|'''Function''' || intracellular iron channeling, biosynthesis of iron-sulfur clusters
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU05750 fra]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=Fra Fra]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=Fra Fra]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 5.377
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 369 bp, 123 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[phoB]]'', ''[[ydhH]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU05750 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU05750 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU05750 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ydhG_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ydhG_621847_622218_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:fra_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU05750]]
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[iron metabolism]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU05750
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| ===Phenotypes of a mutant ===
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| * severe growth phenotype associated with a broadly disturbed iron homeostasis {{PubMed|21744456}}
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU05750&redirect=T BSU05750]
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12184]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' transfers iron onto the B. subtilis SUF system ([[SufU]]-[[SufS]]) for iron-sulfur cluster biosynthesis {{PubMed|21744456}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):''' Fe(2+) is bound with high affinity {{PubMed|20087498}}
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** the protein is active as a dimer {{PubMed|20087498}}
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| ** ([[Fra]])2-[[SufU]], requires iron {{PubMed|20087498}}
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| ** ([[Fra]])-[[HemH]], requires iron, Kd of 1.63 ± 0.02 μM {{PubMed|25826316}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU05750&redirect=T BSU05750]
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2OC6 2OC6]
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/Q797E6 Q797E6]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU05750]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ydhG_621847_622218_1 fra] {{PubMed|22383849}}
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| * '''Sigma factor:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** number of protein molecules per cell (minimal medium with glucose and ammonium): 178 {{PubMed|24696501}}
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| ** number of protein molecules per cell (complex medium with amino acids, without glucose): 451 {{PubMed|24696501}}
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| =Biological materials =
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| * '''Mutant:''' available in [[Mohamed Marahiel]]'s lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed> 20087498 21744456 25826316</pubmed>
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| [[Category:Protein-coding genes]]
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