|
|
| Line 1: |
Line 1: |
| * '''Description:''' branched-chain amino acid transporter, major transporter for isoleucine and valine, may also be involved in threonine transport <br/><br/>
| | {{#bank: BcaP}} |
| {| align="right" border="1" cellpadding="2" | |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''bcaP''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yhdG ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || branched-chain amino acid transporter
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis/acquisition of branched-chain amino acids
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU09460 bcaP]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=BcaP BcaP]'''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49 kDa, 9.735
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1395 bp, 465 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhdF]]'', ''[[yhdH]]''
| |
| |-
| |
| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU09460 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU09460 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU09460 DNA_with_flanks]
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:yhdG_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ctrA_1023350_1024747_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:bcaP_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU09460]]
| |
| |-
| |
| |}
| |
|
| |
|
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[transporters/ other]]}},
| |
| {{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}},
| |
| {{SubtiWiki category|[[utilization of amino acids]]}},
| |
| {{SubtiWiki category|[[membrane proteins]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
| {{SubtiWiki regulon|[[CodY regulon]]}}
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU09460
| |
|
| |
| ===Phenotypes of a mutant ===
| |
| * no phenotype for the single mutant, the triple ''[[bcaP]] [[brnQ]] [[braB]]'' mutant is strongly impaired in the transport of isoleucine and valine at low concentrations {{PubMed|25645558}}
| |
| * resistant to growth inhibition by high concentrations of threonine {{PubMed|25645558}}
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU09460&redirect=T BSU09460]
| |
|
| |
| * '''DBTBS entry:''' no entry
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13013]
| |
|
| |
| === Additional information===
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:'''
| |
| ** high affinity uptake of isoleucine and valine {{PubMed|25645558}}
| |
|
| |
| * '''Protein family:'''
| |
| ** [[APC superfamily of transport proteins|amino acid, polyamine, organocation (APC) superfamily of transporters]], [http://www.tcdb.org/search/result.php?tc=2.A.3 TC 2.A.3]
| |
|
| |
| * '''Paralogous protein(s):''' [[YfnA]]
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
| ** for Ile: Km ≈ 4.1 μM; Vmax at 26°C was ~15 nmol/min/ mg protein {{PubMed|25645558}}
| |
|
| |
| * '''Domains:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''Cofactor(s):'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
|
| |
| * '''[[Localization]]:''' cell membrane [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
| |
|
| |
| === Database entries ===
| |
| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU09460&redirect=T BSU09460]
| |
|
| |
| * '''Structure:'''
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/O07576 O07576]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU09460]
| |
|
| |
| * '''E.C. number:'''
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
|
| |
| * '''Operon:'''
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ctrA_1023350_1024747_1 bcaP] {{PubMed|22383849}}
| |
|
| |
| * '''[[Sigma factor]]:'''
| |
|
| |
| * '''Regulation:'''
| |
| ** repressed during growth in the presence of branched chain amino acids ([[CodY]]) {{PubMed|12618455,21097623}}
| |
|
| |
| * '''Regulatory mechanism:'''
| |
| ** [[CodY]]: transcription repression {{PubMed|12618455,21097623,18083814,21699902}}
| |
|
| |
| * '''Additional information:'''
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:''' BKE09460 (''[[bcaP]]''::''erm'', available in the BGSC and in [[Fabian Commichau]]'s and [[Jörg Stülke]]'s labs)
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
|
| |
| <pubmed>18083814,12618455, 20935095, 18763711, 21097623 21699902 25645558</pubmed>
| |
|
| |
| [[Category:Protein-coding genes]]
| |
